Diabetes alters vascular mechanotransduction data: Pressure-induced regulation of mTor and associated signaling in the rat inferior vena cava

Diabetes is a multifaceted disease with various etiologies. The complexity of this pathology creates a myriad of factors that must be considered when addressing surgical outcomes and prognosis. Of vital importance to cardiovascular surgery is the viability of homographic vein grafts. Due to the fact, diabetic patients have a higher rate of vein graph failure, a greater understanding of the effect diabetes has on vascular mechano-transductive response is critical to improving patient prognosis. This article represents data regarding a study published in Cardiovascular Diabetology (Rice et al., 2006) [1] and Open Journal of Endocrine and Metabolic Diseases (Rice et al., 2015) [2] with the purpose of evaluating the effect of pressurization on rat inferior venae cavae (IVC). Here we provide the information about the method and processing of raw data related to our prior publish work and Data in Brief articles (Rice et al., Submitted for publication) [3,4]. The data contained in this article evaluates the contribution of mTor signaling and associated proteins. IVC from lean and obese animals were exposed to a 30 min perfusion of 120 mm Hg pressure and evaluated for changes in expression and phosphorylation of mTor, p70s6k, GSK3β, and 4EBP-1.


a b s t r a c t
Diabetes is a multifaceted disease with various etiologies. The complexity of this pathology creates a myriad of factors that must be considered when addressing surgical outcomes and prognosis. Of vital importance to cardiovascular surgery is the viability of homographic vein grafts. Due to the fact, diabetic patients have a higher rate of vein graph failure, a greater understanding of the effect diabetes has on vascular mechano-transductive response is critical to improving patient prognosis. This article represents data regarding a study published in Cardiovascular Diabetology (Rice et al., 2006) [1] and Open Journal of Endocrine and Metabolic Diseases (Rice et al., 2015) [2] with the purpose of evaluating the effect of pressurization on rat inferior venae cavae (IVC). Here we provide the information about the method and processing of raw data related to our prior publish work and Data in Brief articles  [3,4]. The data contained in this article evaluates the contribution of mTor signaling and associated proteins. IVC from lean and obese animals were exposed to a 30 min perfusion of 120 mm Hg pressure and evaluated for changes in expression and phosphorylation of mTor, p70s6k, GSK3β, and 4EBP-1.
& Data accessibility Data is presented in this article and is related to articles published and in review [1][2][3][4] Value of the data The data presented in this Brief is vital to understanding the effect of diabetes on tissue. This data gives insight into the how diabetes alters tissue response to stimuli. The data can provide comprehensive analysis of the effect of diabetes on vascular signaling in vein transplant surgery.
These data provides a more thorough understanding of the mTor involvement in pressure mediated signaling in both diabetic and lean IVC.

Animals
All procedures were performed in accordance with the public Health Service policy on animal welfare, Guide for the Care and Use of Laboratory Animals as approved by the Council of the American Physiological Society and the Animal Use Review Board of Marshall University. Rats were obtained from the Charles River Laboratories (Wilmington, MA) (Young (10 week, n ¼12) male normal lean Zucker (LNZ) and young (10 week, n ¼12) male obese syndrome-X Zucker (OSXZ)) and barrier housed one per cage in an AAALAC approved vivarium. Housing conditions included 12H:12H dark-light cycle and temperature was maintained at 2272°C. Food and water were provided ad libitum.

Inferior vena cava preparation
Collection, perfusion of the Inferior venae cavae was performed according to the method described by Rice et al. [1][2][3][4]. In brief Rats were anesthetized with a ketamine-xylazine (4:1) cocktail (50 mg/kg ip), the inferior vena cava was isolated aseptically and the in situ length was obtained. Vessel were mounted in oxygenated Krebs-Ringer bicarbonate (KRB) buffer maintained at 37°C and allowed to equilibrate in the vessel chamber for at least one hour before pressure loading. Mounted vessels were subjected to 120 mm Hg of pressure for 30 min to examine the effect of increased loading on signal transduction in the inferior vena cavae.

Immunoblot analysis
After pressurization, Inferior vena cavae were snap-frozen in liquid nitrogen. Protein isolates were prepared according to the protocol described by Rice et al. [1][2][3][4]. In brief samples were pulverized in liquid nitrogen and proteins were extracted using T-PER, diluted to a concentration of 1.5 mg/mL in SDS-loading buffer, ran on 10% or 15% SDS-PAGE gel, transferred onto Hybond nitrocellulose membranes (Amersham Biosciences, Piscataway, NJ) using standard conditions. Membranes were then probed for the molecules of interest using the protocol outlined by Rice et al. [1][2][3][4], and quantified by densitometry using a flatbed scanner (Epson Perfection 3200 PHOTO) and Imaging software (AlphaEaseFC).

Data analysis
Data were analyzed using the Sigma Stat 3.0 and results were presented as mean 7 SEM. A one-way analysis of variance (ANOVA) was performed for overall comparisons followed by the Student-Newman-Keuls post hoc test to determine differences between groups. The level of significance accepted a priori was r0.05.