Data comparing the plasma levels of procollagen C-proteinase enhancer 1 (PCPE-1) in healthy individuals and liver fibrosis patients

This article provides a protocol for determination of human procollagen C-proteinase enhancer 1 (PCPE-1) concentrations by ELISA. The inter-assay and intra-assay coefficients of variability are given and so are the average plasma concentrations of PCPE-1 in healthy (control) individuals and liver fibrosis patients.

A procedure for determination of human PCPE-1 concentrations by ELISA was developed using a specific monoclonal antibody for immobilization of human PCPE-1 and a polyclonal rabbit antibody against human PCPE-1 for its detection. PCPE-1 concentrations in plasma samples from healthy individuals and liver fibrosis patients were determined using this assay. Data source location Tel Aviv University, Tel Aviv, Israel Data accessibility Data is provided within this article

Value of data
An ELISA method for determination of procollagen C-proteinase enhancer 1 (PCPE-1) concentrations in human plasma is described.
The method can be used for determination of PCPE-1 concentrations in other body fluids.
The data highlights the potential of PCPE-1 as a new non-invasive biomarker of liver fibrosis, which could be valuable clinically.

Data
The data includes two Figures and three Tables. Fig. 1 presents a calibration curve for determination of human PCPE-1 concentrations. Tables 1 and 2 provide the plasma concentrations of PCPE-1 in healthy individuals and liver fibrosis patients, respectively. Table 3 summarizes the clinical features of the patients. Fig. 2 compares the average plasma concentrations of PCPE-1 in healthy individuals and liver fibrosis patients. Supplemental Tables S1 and S2 provide raw data used to calculate the inter-and intra-assay coefficients of variability, respectively. Standard curve for quantification of huPCPE-1 by sandwich ELISA. Increasing amounts of purified recombinant huPCPE-1 were adsorbed to wells pre-coated with a mouse monoclonal antibody to huPCPE-1. Bound PCPE-1 was detected using a rabbit polyclonal antibody to huPCPE-1 and quantified using an APA-conjugated goat anti rabbit IgG antibody. Each value represents mean 7standard deviation (SD); n¼2. mOD, Optical Density at 405 nm expressed in milli units.

Proteins and antibodies
Human recombinant PCPE-1 (huPCPE-1) was produced in 293-EBNA cells and purified from conditioned culture media as described [1,2]. Monoclonal antibody 7A11/1 to huPCPE-1 was produced in our laboratory and is available commercially (Sigma, Santa Cruz). Rabbit polyclonal antibody to human recombinant PCPE-1 was prepared in our laboratory and its IgG fraction isolated from the serum using standard protocols. Similar polyclonal antibodies are available commercially (AssayPro, Proteintech etc.) and can be used instead. Alkaline phosphatase (APA) conjugated goat antibody to rabbit IgG, a monoclonal antibody against the Flag peptide (M2), and bovine serum albumin (RIA grade; cat # A7888) were from Sigma.

Experimental design
Experiments were approved by the Sheba Medical Center ethics committee (SMC-9650-12). Five liver cirrhosis patients at ages 42-64 years old and five healthy individuals at ages 28-56 years old were randomly selected. All of the participating individuals provided written informed consent.

Preparation of plasma samples
Blood was drown into plastic Citrate tubes (BD). After one to two hours at room temperature, the tubes were centrifuged (2000g; 15 min; room temperature) and plasma was transferred into Eppendorf tubes. Plasma samples were divided into aliquots and stored at −80°C until use, avoiding repeated thawing and freezing.

Statistical analysis
Statistical significance was evaluated using two-tailed independent t-test. P value o0.05 was considered statistically significant.

Transparency document. Supporting information
Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2017.08.047.