Dataset on antitumor properties of silver nanoparticles from Gloriosa superba (L.) seed on Dalton Lymphoma Ascites (DLA) tumor: Facile and biocompatible approach

The dataset depicted in this article related to our earlier article entitled “Phytofabrication and encapsulated of silver nanoparticles from Gloriosa Superba” (Saradha Devi et al., 2017) [1], which reports the characteristic features (UV Visible spectra, FTIR, SEM, TEM, DLS, Zeta potential and XRD analysis) of the Gloriosa superba biosynthesised silver nanoparticles (AgNPs). In this context, the present dataset was provided to identify the antioxidant, antitumor and apoptotic (in DLA cells) properties with the synthesized AgNPs. The result enlightens the AgNPs exhibits antitumor, apoptotic activity in DLA cells and antioxidant properties. The results of the in vivo experiments increased life span of liver cells in DLA induced tumour mice and not showed any histopathological variations between control and DLA induced mice animals. The HPTLC examination of the Gloriosa superba (L.) seed extract infers the presence of colchicines derivatives as a major alkaloid sources.


Specifications
Data accessibility Data provided as graph and accessible within article

Value of data
The HPTLC data displays the colchicine at 254 nm (peak 9) and the 13 unknown peaks in seed extract ( Fig. 1b-e).
The antioxidant property of the seed extract and AgNPs are comparable with the vitamin C (Fig. 2). The flow cytometry data analysis confirms the apoptotic nature of the seed extract and AgNPs to DLA cells ( Fig. 3a and b) The MTT assay data shows the toxicity to the DLA cell lines with IC 50 values of 29 µg and 21 µg for seed extract and for AgNPs respectively.
The data analysis of the trypan blue assay exhibited the concentration dependent cytotoxic effect in DLA tumor cells with the seed extract and AgNPs ( Fig. 3c and d).

Data
The dataset depicted in this article related to our earlier article entitled "Phytofabrication and encapsulated of silver nanoparticles from Gloriosa superba" [1]. The free radicle scavenging properties of the G. superba seed extract and AgNPs were represented by DPPH, hydroxyl radicle, superoxide radicle, hydrogen peroxide and nitric oxide assays. The free radical scavenging efficacy of AgNPs was found to be higher than that of G. superba seed extract [2]. The toxicity effect of the seed extract and AgNPs on DLA tumor cells was carried out with MTT assay [3,4]. The data analysis of the in vitro anticancer property of G. superba seed extract and AgNPs were carried out with the DLA cell lines. The in vivo antitumor data study shows the average life span of DLA tumor bearing mice identified as 20 days, but the seed extract and AgNPs administrated animals were increased the life span up to 72 and 75 days respectively (data not shown) [5]. The histopathological data of liver section of treated and controls groups showed normal lobular architecture with intact central vein and sinusoids and normal portal triad architectures (Fig. 4).

Preparation of the extract and synthesis of silver nanoparticles
Gloriosa superba seeds were collected from outskirts of Thanjavur district, Tamil Nadu province in India and authenticated. The 10 g of seeds powder was extracted with 150 ml of methanol. Then 10 ml of methanol seed extract was mixed with 90 ml of 1 mM silver nitrate solution and incubated for a period of 15 h at room temperature. The color changed from yellowish brown to dark brown color indication the formation of silver nanoparticles (Fig. 1a).

in vitro antioxidant activity
Antioxidant analysis of methanol extract of G. superba seed and AgNPs was evaluated by assessing their scavenging of DPPH, hydroxyl radical (OH.), Superoxide radical (.O2)− radicals and non-radicals such as Hydrogen peroxide (H 2 O 2 ) and Nitric oxide (NO) against the standard Vitamin C by standard protocols [6].

Maintenance of animals
Swiss albino mice of 5-7 weeks old (20-25 g) were bought from Animal breeding station, Kerala Agricultural University, Thrissur, India. The mice were maintained under dark/light cycle (14/10 h). The mice were acclimatized to laboratory conditions for 15 days before the commencement of the experiments. All procedures described were reviewed and approved by the University Animals Ethical Committee (Reg no: 623/02/b/CPCSEA).

in vitro antitumor activity
in vitro antitumor activity of methanol extract of G. superba seed and AgNPs was evaluated by assessing their cytotoxic effect to DLA tumor cells by MTT proliferation assay, Trypan blue exclusion assay and Apoptotic measurement activity [7].

in vivo antitumor activity
in vivo cytotoxic studies were carried out using the ED 50 of methanol extract of G. superba seed and AgNPs to follow the antitumor activity in terms of increase in life Span and histological examination of seven groups (Groups A to G) of (6 mice/group) Swiss albino mice transplanted with DLA tumor cells [8].
Grouping of animals. The Swiss albino mice were divided into 7 groups with six animals in each treatment groups as follows: Group A: Mice received 100 μl of PBS and served as a control;. Group B: Mice received 100 μl of DMSO and served as vehicle control. Group C: Mice received methanolic extract (33 μg in 100 μl (ED 50 ) of DMSO).  Group E: Mice received one acute dose of 1×10 6 DLA tumour cells in 100 µl of PBS on the first day of the experimental period and also ED 50 dose of methanolic extract, throughout the experimental tenure.
Group F: Mice received one acute dose of 1×10 6 DLA tumour cells in 100 µl of PBS on the first day of the experimental period and also ED 50 dose of AgNPs, throughout the experimental tenure.
In Group G: Mice, tumour was induced by the administration of one acute dose of 1× 10 6 DLA tumour cells in 100 µl of PBS on the first day of the experimental period.
The experiments were carried out for 20 days and 60 days. At the end of the study the mice were sacrificed after an overnight fasting. The liver was dissected, blotted of blood and washed with PBS of pH 7.2. The histological examination of the liver of all the experimental mice was carried out.

High performance thin layer liquid chromatography
The seed extract of G. superba was dissolved with HPLC grade methanol 100 mg/0.5 ml. The samples loaded plate was kept in TLC twin trough developing chamber with respective mobile phase (Ethyl acetate-Methanol-Water 10:1.35:1) up to 90 mm and captured the images at Visible light, UV 254 nm and UV366 nm [9].