Data regarding the growth of Lactobacillus acidophilus NCFM on different carbohydrates and recombinant production of elongation factor G and pyruvate kinase

The present study describes the growth of the very well-known probiotic bacterium Lactobacillus acidophilus NCFM on different carbohydrates. Furthermore, recombinant production of putative moonlighting proteins elongation factor G and pyruvate kinase from this bacterium is described. For further and detailed interpretation of the data presented here, please see the research article “Mucin- and carbohydrate-stimulated adhesion and subproteome changes of the probiotic bacterium Lactobacillus acidophilus NCFM” (Celebioglu et al., 2017) [1].


Subject area
Biology More specific subject area

Microbiology, Biochemistry
Type of data Bacterial cells were grown on different carbohydrates until stationary phase (24 h) in batch cultures. Genes encoding elongation factor G and pyruvate kinase were cloned and recombinantly produced in Escherichia coli cells.

Experimental features
Growth was measured at 600 nm at the stationary phase (24 h). Recombinant elongation factor G and pyruvate kinase were purified using HisPur™ Cobalt Purification Kit (Thermo Scientific), followed by SDS-PAGE to visualize the purified proteins.

Data source location
Technical University of Denmark, Kgs. Lyngby, Denmark Data accessibility All data are presented in this article

Value of the data
Growth data presented here shows growth potential of the probiotic bacterium Lactobacillus acidophilus NCFM on different carbon sources.
The growth data may be used by researchers to understand the ability of the bacterium to grow on different carbon sources.
Data regarding recombinant production of elongation factor G and pyruvate kinase from Lactobacillus acidophilus NCFM may be used by researchers to apply the same procedure.
Data shown here and in Ref. [1] are useful for the researchers who are working on gut microbiota, probiotic bacteria, carbohydrate-microbe interactions, and moonlighting proteins.

Data
The extensively used probiotic bacterium Lactobacillus acidophilus NCFM was grown on nine different carbohydrates including growth on glucose supplemented with mucin ( Fig. 1). The putative moonlighting proteins elongation factor G and pyruvate kinase also identified from this bacterium by differential proteomics [1] were recombinantly produced using the primers shown in Table 1 and purified by his-tag affinity chromatography. The purified proteins were analysed by SDS-PAGE (Fig. 2). Table 2 reports on the mass spectrometric identification of the two purified proteins without any identification of E. coli proteins.