Data of added-value lipid production, Arachidonic acid, among other lipids by Mortierella elongata, using low cost simulated wastewater

This article presented an innovative data of feasibility to produce Arachidonic acid (ARA), as added-value Polyunsaturated Fatty Acids (PUFA), among other lipids from Mortierella elongata, using simulated low cost sugarcane wastewater, vinasse, as a carbon source. Data from lipids quantification by total lipids extraction and by lipid classes was presented. M. elongata was able to produce 156.45mg of ARA per g of total lipids.


Subject area
Biology More specific subject area

Lipids production by biotechnological process and their extraction
Type of data Table, text file, graph, figure How data was acquired Gas Chromatography -Flame Ionization Detector (GC-FID)

Data format Raw data collection and analysis Experimental factors
Biomass production and oven drying and lipids extraction, classification, transesterification and analyzed in the GC-FID.

Experimental features
The microorganism was cultivated in low cost simulated sugarcane wastewater, vinasse.

Data source location
Mortierella elongata (CBS 121.71) was acquired from the CBS-KNAW Fungal Biodiversity Centre, Netherlands. All experiments were developed in Campinas-SP, Brazil. Data accessibility Data are presented in this article

Value of the data
The obtained data are innovative and important in the research area of biotechnology providing results of lipids production by M. elongata, a fungus, using low cost substrate, sugarcane wastewater vinasse, applied in Nutrition and Pharmaceutical Industry.
Sugarcane vinasse, the main residue of ethanol process production, is a promising low cost carbon source and produced in large amounts. Since there is estimative of a growth in Brazilian domestic ethanol consumption of 58.8 billion liters by 2019 [1], with a corresponding vinasse production of 588 billion liters.
The data can be most useful for the researcher, research student, academician and industry to acquire innovative process development and production.
The described data identify a sustainable mechanism and with low cost to produce added-value lipid mainly, Arachidonic acid, since it is recommend injection of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and Arachidonic acid (ARA) in the infant [2], and for adults prevent coronary heart disease [3] and cancer [4].

Data
Mortierella elongata is an oleaginous fungus able to accumulate lipids. The data of lipids extraction from M. elongata are presented in the Table 1. The total lipids extraction from M. elongata were 14.95% and 8.54%, by MC and HIP method, respectively, using simulated low cost sugarcane wastewater, vinasse as a carbon source. Triacylglycerol (TGA) was highest lipid class observed, which is in accordance with previous studies for genus Mortierella, using other substrate [5]. Considering the HIP and MC lipids extraction process, the mixture Hexane/Isopropanol has less polar lipid interaction properties than Chloroform/Methanol [6], which reduce the extraction of phospholipids as observed in the data presented in the Table 1.
According to Fig. 1 (Chromatograms in the Supplementary data), it is presented the data profile of lipids by chain length and saturation, since C18:0, C18:1 and C20:4, the Arachidonic acid (ARA) were the predominant lipids. The Fig. 2 shows data of percentage and amount of ARA extracted by lipid class. In HIP method there was a high ARA extraction by percentage (26.0 %) of total fatty acids and in recovery weight (156.45mg of ARA g-1 of TGA and 168.81mg of ARA g-1 of TL) in five days of culture (Fig. 2), using simulated vinasse as a carbon source.  TAGtriacylglycerol, NEFAfree fatty acids, PP -phospholipids, TLtotal lipids, DWdry weight.

Microorganism cultivation
Mortierella elongata (CBS 121.71) was inoculated per 5 days, at 29°C, in shaker at 200rpm, with culture medium simulating the main carbon sources of sugarcane vinasse [7], main sub-product of ethanol process production.

Lipids quantification
The stratified total lipids and by class were transesterified with NaOH-methanol, Boron Trifluoride, and Hexane. The Fatty Acids Methyl Ester (FAME) were analyzed in the GC-FID (Chrompack 9001) using CP-Sil 88 capillary column (Chromatograms in the Supplementary data).