Raw data of the effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity

The raw data showed in this article comes from the published research article entitled “Protective effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity” Food Chem Toxicol. 2017 May 3. pii: S0278-6915(17)30226-0. DOI:10.1016/j.fct.2017.04.048. [1]. Data illustrates antitoxic and antigenotoxic effects of Chlorogenic acid (CGA) on toxicity and genotoxicity produced by the in vivo treatment with mitochondria toxin 3-Nitropropionic acid (3-NP) in mice. Toxicity and genotoxicity was evaluated in erythrocytes of peripheral blood through the micronuclei assay. Data was share at the Elsevier repository under the reference number FCT9033.


Subject area
Biology More specific subject area

Toxicology
Type of data Tables  How data was  acquired Data from erythrocytes from peripheral blood, stained with H&E were classified in Normochromatic, Polychromatic or Polychromatic with micronuclei cells. 1000 cells per each condition were counted with the aid of by the aid of an optic microscope (Leica, Microsystems AG).

Data format
Raw data Experimental factors In order to determine toxicity and genotoxicity of 3-NP, as well as protective effects of CGA, 6 experimental groups were evaluated; Negative control, Control (PB), 3-NP, CGA, 3-NP þ CA, P/CA, 3-NP þ CA and P/CA, 3-NP Each treatment lasted for 5 days except those where 5 days of pretreatment was present.

Experimental features
To evaluate toxic and genotoxic effect of 3-NP and the antitoxic and antigenotoxic effects of CGA in erythrocytes.

Data source location
México City, México Data accessibility Data are available in this article and were place also in a public repository provided by Elsevier submission system, under reference number FCT9033 Value of the data Data displays Normochromatic, Polychromatic or Polychromatic cells with micronuclei in the different experimental conditions and can be used by other research groups.
Toxicity and Genotoxicity were measured in peripheral blood by using the micronuclei assay. Τhese data are important because few studies have carried out to evaluate toxicity and genotoxicity of 3-NP which can be found in plants like sugar cane that are eaten by cattle and humans.
Data about CGA protective effects are important because CGA is found in a variety of food products which can help to protect the health of living organisms.

Data
Raw data presented in this paper gives information about protective role of CGA on toxic and genotoxic effects of 3-NP in erythrocytes from peripheral blood. Data are shown in Tables 1-7 which illustrate the effect in each evaluated time.
All groups were treated for 5 days with i.p. doses of 3-NP (15 mg/kg), CGA (100 mg/kg).   Extraction and Characterization of CGA: Aerial parts of B. scordioides (300 g) were dried, ground and extracted with hexane and methanol in succession. The methanolic portion was evaporated under reduced pressure at 55°C to obtain a syrup residue (30 g). CGA was isolated from methanolic extract by open column chromatography using SiO2 [2].
Micronuclei Assay: Samples of peripheral blood were obtained from the mice caudal vein at 24 h, 48 h, 72 h, 96 h, 120 h and 144 h after starting each treatment in each experimental group. A drop of blood was placed on a glass slide (3 slides per mice) and fixed with methanol for further H&E staining for 10 min.