Data on the fate of MACS® MicroBeads intramyocardially co-injected with stem cell products

The data presented in this article are related to the research article “Intramyocardial Fate and Effect of Iron Nanoparticles co-injected with MACS® purified Stem Cell Products” (Müller et al., 2017) [1]. This article complements the cellular localization of superparamagnetic iron dextran particles (MACS® MicroBeads) used for magnetic activated cell sorting (MACS®). Data evaluate the time-dependent detachment of these nanoparticles from CD133+ haematopoietic stem cells (HSCs) and CD271+ mesenchymal stem cells (MSCs). Furthermore, the influence of these stem cells as well as of nanoparticles on cardiac remodeling processes after myocardial infarction (MI) was investigated.


a b s t r a c t
The data presented in this article are related to the research article "Intramyocardial Fate and Effect of Iron Nanoparticles co-injected with MACS s purified Stem Cell Products"   [1]. This article complements the cellular localization of superparamagnetic iron dextran particles (MACS s MicroBeads) used for magnetic activated cell sorting (MACS s ). Data evaluate the timedependent detachment of these nanoparticles from CD133 þ haematopoietic stem cells (HSCs) and CD271 þ mesenchymal stem cells (MSCs). Furthermore, the influence of these stem cells as well as of nanoparticles on cardiac remodeling processes after myocardial infarction (  Data can be useful for other researchers analyzing the cardiac regeneration potential of MACS s purified stem cells products.
Data clarifies the safety of MACS s MicroBeads for clinical application.

Data
The data include information about the cellular localization of MACS s MicroBeads (labelled with Labeling Check Reagent-FITC) right after the manual MACS s based isolation of CD133 þ and CD271 þ stem cells (Fig. 1). The detachment of FITC-labelled MACS s MicroBeads was evaluated by measuring the time-dependent fluorescence intensity of MACS s purified CD133 þ cells incubated under cell culture conditions (37°C in StemSpan TM H3000) using flow cytometry (Fig. 2). Furthermore, the effect of manually and automatically (Good Manufacturing Practice (GMP)-conform) MACS s purified CD133 þ and CD271 þ stem cells as well as of MACS s MicroBeads on fibrosis after MI was assessed in a cardiac ischemia/reperfusion mouse model by histological staining (Fig. 3).

Microscopic analysis
For staining, cells were incubated with Labeling Check Reagent-FITC (Miltenyi Biotec) and CellMask TM Plasma Membrane Stains (Thermo Fisher Scientific, Schwerte, Germany). Subsequently, samples were mounted with Fluoroshield™ with DAPI (Sigma-Aldrich, Taufkirchen, Germany) on microscope slides. To evaluate the localization of MACS s MicroBeads, labelled cells were subjected to three-dimensional structured illumination microscopy (SIM) using the ELYRA PS.1 LSM 780 system (Carl Zeiss, Jena, Germany). Images were acquired as z-stacks and processed with ZEN software (Carl Zeiss). Final images were obtained by creation of maximum projections.

Histological investigations
Three weeks after cardiac surgery, murine hearts were embedded in Tissue-Tek s O.C.T. TM Compound (Zoeterwoude, Netherlands) and snap-frozen. To investigate fibrosis, 5 mm thick slices were cut from two different horizontal myocardial infarction levels and stained with Sirius Red (Division Chroma, Muenster, Germany) and Fast Green FCF (Sigma-Aldrich). Sirius Red positive regions (indicating collagen deposition) were examined in the infarction border zone (BZ) in five randomly chosen fields (each per section; one section per level; 400 Â ) using computerized planimetry.