Data supporting the anticancer activity of posterior salivary gland (PSG) toxin from the cuttlefish Sepia pharaonis Ehrenberg (1831)

The data presented illustrated the in vitro anti-proliferative effect of the PSG toxin from the cuttlefish, Sepia pharaonis. The cytostatic potentials of the PSG toxin were determined by the lymphocyte migration inhibition assay. The PSG toxin (50 μg/ml) exhibited commendable inhibition of the migration of lymphocytes across the agarose gel matrix under the presence of lipopolysaccharide mitogen, with a mean migration index of 0.625. The cytotoxicity of the PSG toxin against selected cancer cell lines was determined using the MTT assay. The PSG toxin exhibited dose-dependent cytotoxicity against the MCF-7 breast cancer cells followed by KB (oral), HeLa (cervical) and A549 (lung) cancer cell lines. The PSG toxin also exhibited proportional release of LDH leakage by mitochondrial damage with an IC50 of 13.85 μM against MCF-7 breast cancer cells. The in vitro anticancer activity of the PSG toxin against the selected cell lines was evaluated by Karthik et al. (2017) [1].


a b s t r a c t
The data presented illustrated the in vitro anti-proliferative effect of the PSG toxin from the cuttlefish, Sepia pharaonis. The cytostatic potentials of the PSG toxin were determined by the lymphocyte migration inhibition assay. The PSG toxin (50 μg/ml) exhibited commendable inhibition of the migration of lymphocytes across the agarose gel matrix under the presence of lipopolysaccharide mitogen, with a mean migration index of 0.625. The cytotoxicity of the PSG toxin against selected cancer cell lines was determined using the MTT assay. The PSG toxin exhibited dose-dependent cytotoxicity against the MCF-7 breast cancer cells followed by KB (oral), HeLa (cervical) and A549 (lung) cancer cell lines. The PSG toxin also exhibited proportional release of LDH leakage by mitochondrial damage with an IC 50 of 13.85 μM against MCF-7 breast

Value of the data
The provided data demonstrates the cytostatic potentials of the PSG toxin from S. pharaonis against peripheral blood mononuclear leucocytes.
The data provided illustrates the commendable anti-proliferative action of the PSG toxin from S. pharaonis against the selected cancer cell lines.
The data might be valuable to researchers interested in anti-proliferative action of toxins from marine mollusks.
The data might also be of value to researchers investigating the anti-proliferative and anticancer activity of marine bioactive compounds.

Data
The migration of the lymphocytes was inhibited by the PSG toxin (5, 25 and 50 mg/ml) with a mean diameter of 1.65 70.55 mm and 1.42 7 0.64 mm respectively. The lymphocytes exhibited pronounced migration under the influence of LPS with a mean diameter of 3.45 70.83 mm (Fig. 1).
The cell viability curve of the PSG toxin against the KB oral cancer cells with an IC 50 concentration of 32.5 mM. The purified PSG toxin also exhibited significant inhibition against the proliferation of HeLa cervical cancer cells and A549 lung cancer cells at an IC 50 value of 16.5 mg/ml (23.2 mM) and 22.45 mg/ml (31.65 mM) (Fig. 2). The inlet figure shows the cell viability in (A) control, (B) PSG toxin (100 μg/ml) and (C) Paclitaxel treated cells showing the zones of apoptosis.
The PSG toxin also exhibited commendable LDH leakage against KB oral cancer cells, HeLa cervical cancer cells and A549 lung cancer cells with IC 50 concentrations of 22.8 μg/ml, 17.45 μg/ml and 23.52 μg/ml respectively (Fig. 3).

Lymphocyte migration inhibition assay
The cytotoxicity of the purified PSG toxin against the primary cells peripheral blood mononuclear cells (PBMC) was determined using the lymphocyte migration inhibition assay following the method of Mousseau et al. (2007). The inhibition of leucocyte migration treated with PSG toxin (5,10,50 mg/ml) under the influence of mitogen (lipopolysaccharide) was measured using a microscope ruler [2].

in vitro anticancer activity by MTT assay
The cytotoxicity of the purified PSG toxin against selected cancer cell lines was determined by the MTT cell viability assay [3]. The anti-proliferative potentials of the PSG toxin (0.5,1,5,10,25,50 mg/ml) was studied against the adherent cultures of KB (oral), HeLa (cervical) and A549 (lung) cancer cell line and the inhibitory concentrations (IC 50 ) were determined.

LDH release assay
The cell viability and membrane permeability of the PSG toxin (0.5,1,5,10,25, 50 mg/ml) against the KB, HeLa and A549 cancer cells was determined using the LDH leakage assay [4]. The inhibitory effect of the PSG toxin on the mitochondrial enzymes, dehydrogenases are evaluated by the levels of LDH released into medium after action on the substrate lactate in the presence of NADH.