Data on the effect of in vivo knockdown using artificial ErbB3 miRNA on Remak bundle structure

Mature Schwann cells, the peripheral nervous system (PNS) glial cells, have two major roles for neuronal axons (Bunge, 1993) [1]. For large diameter axons, Schwann cells form myelin sheaths with multiple layers. For small diameter axons, they form Remak bundle composed only of single layer of the Schwann cell plasma membrane. In the PNS, ErbB3 forms a dimer with ErbB2 on the Schwann cell plasma membrane. ErbB3 plays a key role in myelination by myelinating Schwann cells, that is to say, its role in myelin thickness. Herein we provide the data regarding the effect of in vivo knockdown of ErbB3 on the thickness between an axon and a neighboring axon in Remak bundle, which is formed by non-myelinating Schwann cells. Since ErbB3 knockout mice are embryonically lethal, Schwann cell lineage-specific transgenic mice transcribing ErbB3 shRNA with an artificial miRNA backbone were generated and used in these experiments (Torii et al., 2014) [2].


a b s t r a c t
Mature Schwann cells, the peripheral nervous system (PNS) glial cells, have two major roles for neuronal axons (Bunge, 1993) [1]. For large diameter axons, Schwann cells form myelin sheaths with multiple layers. For small diameter axons, they form Remak bundle composed only of single layer of the Schwann cell plasma membrane. In the PNS, ErbB3 forms a dimer with ErbB2 on the Schwann cell plasma membrane. ErbB3 plays a key role in myelination by myelinating Schwann cells, that is to say, its role in myelin thickness. Herein we provide the data regarding the effect of in vivo knockdown of ErbB3 on the thickness between an axon and a neighboring axon in Remak bundle, which is formed by non-myelinating Schwann cells. Since ErbB3 knockout mice are embryonically lethal, Schwann cell lineage-specific transgenic mice transcribing ErbB3 shRNA with an artificial miRNA

Value of the Data
This data set is of value to the scientific community to need the information for the biological effect of a receptor tyrosine kinase.
The data provide the valuable information for the role of a receptor tyrosine kinase in the nervous system.
The data allow us to promote our understanding of how a receptor tyrosine kinase contributes to Schwann cell development, especially to the thickness between an axon and a neighboring axon in Remak bundle.
The data provide the valuable information for analyzing the role of target molecules using transgenic mice.

Data
The data shared in this article provide electron microscopic analyses of sciatic nerve's Schwann cells in Schwann cell lineage-specific ErbB3 shRNA transgenic mice (ErbB3 knockdown mice). The data shared also provide electron microscopic analyses of neuronal fibers in transgenic mice.

Data from ErbB3 shRNA transgenic mice
While myelin is composed of multiple layer plasma membranes of myelinating Schwann cells, Remak bundle is axonal one with single layer of the plasma membranes of non-myelinating Schwann cells [1]. In Schwann cell lineage-specific ErbB3 shRNA transgenic mice (

Electron microscopy
Sciatic nerves (2-month-old) were fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.1% cacodylate buffer. The tissues were postfixed with buffered 2% osmium tetroxide, dehydrated with an ethanol gradient, treated with acetone, and embedded in epoxy resin. Ultrathin cross sections, using Cryostar NX70 (Thermo Fisher Scientific), were stained with uranyl acetate and lead citrate. They were observed and photographed with Hitachi electron microscopes [5]. In order to unify the morphologies of Remak bundles in cross sections, we sliced middle portions of sciatic nerves in three mice and calculated the statistical data.

Immunoblotting
The lysates from 2-month-old sciatic nerve tissues were denatured and then separated on sodium dodecyl sulfate-polyacrylamide gels. The electrophoretically separated proteins were transferred to PVDF membranes, blocked with Blocking One reagent (Nacalai Tesque), and immunoblotted first with primary antibodies and then with peroxidase-conjugated secondary antibodies. The bound antibodies were detected using Nacalai Tesque's chemiluminescence reagent. Anti-ErbB3 and anti-actin (control actin) antibodies were obtained from Cell Signaling Technology and MBL, respectively. At least three experiments were carried out under each condition, and a representative bot is shown in the figure.

Ethics statement
Genetically modified/unmodified mice were maintained in accordance with a protocol approved by the Japanese National Research Institute for Child Health and Development Animal Care Committee. They were also maintained in accordance with a protocol approved by the Tokyo University and Pharmacy and Life Sciences Animal Care Committee.

Transparency document. Supporting information
Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2017.04.014.