Circulating growth factors data associated with insulin secretagogue use in women with incident breast cancer

Oral drugs stimulating insulin production may impact growth factor levels. The data presented shows the relationship between pre-existing insulin secretagogues use, growth factor profiles at the time of breast cancer diagnosis and subsequent cancer outcomes in women diagnosed with breast cancer and type 2 diabetes mellitus. A Pearson correlation analysis evaluating the relationship between growth factors stratified by diabetes pharmacotherapy and controls is also provided.

Tumor registry query was followed by vital status ascertainment, and medical records review Luminex s -based quantitation of growth factors (epidermal growth factor, fibroblast growth factor 2, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor BB, and tumor growth factor-β) from plasma samples was conducted.
A Luminex s 200 TM instrument with Xponent 3.1 software was used to acquire all data Data format Analyzed Experimental factors Growth factors were determined from the corresponding plasma samples collected at the time of breast cancer diagnosis Experimental features The dataset included 97 adult females with diabetes mellitus and newly diagnosed breast cancer (cases) and 194 matched controls (breast cancer only). Clinical and treatment history were evaluated in relationship with cancer outcomes and growth factor profiles. A growth factor correlation analysis was also performed. Data source location United States, Buffalo, NY -42°53' 50.3592"N; 78°52' 2.658"W

Data accessibility
The data is with this article

Value of the data
This dataset shows the observed relationship between baseline insulin secretagogues use, circulating growth factor levels at the time of cancer diagnosis and breast cancer outcomes.
Reported data may guide future studies evaluating pharmacotherapy-induced growth factor modulation in breast cancer.
These observations can assist future study design in evaluating the relationship between diabetes pharmacotherapy safety and circulating growth factors levels at the time of cancer diagnosis.

Data
Reported data represents the observed association between use of insulin secretagogues preceding breast cancer and the growth factor profiles at the time of cancer diagnosis in women with diabetes mellitus (Table 1). Data in Table 2 includes the observed correlations between growth factors stratified by type 2 diabetes mellitus pharmacotherapy and controls. C-peptide correlation with each of the studied growth factors is presented in Table 2, however details regarding its determination from plasma, association with cancer outcomes and insulin secretagogues use has been already reported by us [2].   Table 2 Growth factor correlations by secretagogues use.

Experimental design, materials and methods
Evaluation of growth factor profile association with insulin secretagogue use and BC outcomes was carried out under two protocols approved by both Roswell Park Cancer Institute (EDR154409 and NHR009010) and the State University of New York at Buffalo (PHP0840409E). Demographic and clinical patient information was linked with cancer outcomes and growth factor profiles of corresponding plasma specimen harvested at BC diagnosis and banked in the Roswell Park Cancer Institute Data Bank and Bio-Repository.

Study population
As described in the original research article by Wintrob et al. [1], all incident breast cancer cases diagnosed at Roswell Park Cancer Institute (01/01/2003-12/31/2009) were considered for inclusion (n ¼2194). Medical and pharmacotherapy history were used to determine the baseline presence of diabetes.

Inclusion and exclusion criteria
Inclusion criteria were as follows: minimum 18 years of age at diagnosis, presence of pre-existing diabetes at breast cancer diagnosis, and having available banked treatment-naïve plasma specimens in the Institute's Data Bank and Bio-Repository. That is, the blood had to be collected prior to the initiation of any cancer-related therapy (surgery, radiation or pharmacotherapy).
Subjects were excluded if they were male, had prior cancer history or unclear date of diagnosis, incomplete clinical records, type 1 or unclear diabetes status. For a specific breakdown of excluded subjects, please see the original research article by Wintrob et al. [1].
A total of 97 female subjects with breast cancer and baseline diabetes mellitus were eligible for inclusion in this analysis.

Control-matching approach
Each of the 97 adult female subjects with breast cancer and diabetes mellitus (defined as "cases") was matched with two other female subjects diagnosed with breast cancer, but without baseline diabetes mellitus (defined as "controls"). The following matching criteria were used: age at diagnosis, body mass index category, ethnicity, menopausal status and tumor stage (as per the American Joint Committee on Cancer). Some matching limitations applied [1]. Significant correlations are displayed in bolded text. The differences that are only significant in either adjusted or unadjusted correlations are further denoted by an outline. Epidermal growth factor (EGF), fibroblast Growth Factor 2 (FGF-2), hepatocyte growth factor (HGF), platelet-derived growth factor BB (PDGF-BB), tumor growth factor (TGF), vascular endothelial growth factor (VEGF).

Demographic and clinical data collection
Clinical and treatment history was documented by medical chart review. Vital status was obtained from the Institute's Tumor Registry, a local database updated biannually with data obtained from the National Comprehensive Cancer Networks' Oncology Outcomes Database. Outcomes of interest were overall survival (death from breast cancer) and disease-free survival (breast cancer recurrence and/or death). Mean overall and disease-free survival were 113.3 and 107.3 months respectively, both with a minimum follow-up of 25.6 months. For additional details concerning data collection, specific definitions regarding censoring and drug use (including the number of insulin users per analyzed group), and a comprehensive demographic report, please see the original article by Wintrob et al. [1].

Plasma specimen storage and retrieval
All the plasma specimens retrieved from long-term storage were individually aliquoted in color coded vials labeled with unique, subject specific barcodes. Overall duration of freezing time was accounted for all matched controls ensuring that the case and matched control specimens had similar overall storage conditions. Only two instances of freeze-thaw were allowed between biobank retrieval and biomarker analyses: aliquoting procedure step and actual assay.

Luminex s assays
A total of 6 biomarkers (epidermal growth factor, fibroblast growth factor 2, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor BB, and tumor growth factorβ) were quantified according to the manufacturer protocol. The following Luminex s biomarker panels were utilized in this study: TGFB-64K (tumor growth factor-β), HCYTOMAG-60K (platelet-derived growth factor BB), and HAGP1MAG-12K (epidermal growth factor, fibroblast growth factor 2, vascular endothelial growth factor, and hepatocyte growth factor) produced by Millipore Corporation, Billerica, MA. C-peptide determinations were done according to the manufacturer protocol as previously reported [2].

Biomarker-pharmacotherapy association analysis
Biomarker cut-point optimization was performed for each analyzed biomarker. Biomarker levels constituted the continuous independent variable that was subdivided into two groups. Cut-point selection was determined by p-value optimization using the log rank method with respect to survival (both overall and disease-free) as the dependent variable with the condition of a minimum biomarker group size of 10 patients. The results of this analysis yielded the cut-point for each biomarker that would provide the most significant separation of a Kaplan-Meier survival probability curve by assigning the subject to their respective biomarker category, specifically above or below the identified cut-point. Thus identifying potential biomarker ranges associated with poorer outcomes, specifically, ranges associated with a lower survival probability. Quartiles were also constructed. The resultant biomarker categories were then ztested for association with type 2 diabetes mellitus therapy and controls by Fisher's exact test. The continuous biomarker levels were also tested for association with diabetes therapy and controls across groups by the Kruskal-Wallis test and pairwise by the Wilcoxon rank sum. Multivariate adjustments were performed accounting for age, tumor stage, body mass index, estrogen receptor status, and cumulative comorbidity. The biomarker analysis was performed using R Version 2.15.3. Please see the original article for an illustration of the analysis workflow [1].
Correlations between biomarkers stratified by type 2 diabetes mellitus pharmacotherapy and controls were assessed by the Pearson method. Correlation models were constructed both with and without adjustment for age, body mass index, and the combined comorbidity index. Correlation analyses were performed using SAS Version 9.4.

Funding Sources
This research was funded by the following grant awards: Wadsworth Foundation Peter Rowley Breast Cancer Grant awarded to A.C.C. (UB Grant number 55705, Contract CO26588).