Data on interleukin (IL)-2- and IL-15-dependent changes in IL-2Rβ and IL-2Rγ complexes

We provide detailed datasets from our analysis of the proteins that associate with IL-2Rβ and IL-2Rγ in T-cells stimulated with IL-2 or IL-15 compared with resting T-cells, as identified by SILAC-based quantitative proteomics. We also include quantitative data regarding site-specific phosphorylation events observed both in IL-2Rβ and IL-2Rγ. Moreover, we provide results demonstrating the specific protein recruitment capacity of four of those site-specific phosphorylations. The proteomics and phosphoproteomics data described in this article is associated with a research article entitled “Characterization of receptor-associated protein complex assembly in Interleukin (IL)-2- and IL-15-activated T-lymphocytes” (Osinalde et al., 2016 [1]). The mass spectrometry data have been deposited to the ProteomeEXchange Constorium with the identifier PXD002386.


b s t r a c t
We provide detailed datasets from our analysis of the proteins that associate with IL-2Rβ and IL-2Rγ in T-cells stimulated with IL-2 or IL-15 compared with resting T-cells, as identified by SILAC-based quantitative proteomics. We also include quantitative data regarding site-specific phosphorylation events observed both in IL-2Rβ and IL-2Rγ. Moreover, we provide results demonstrating the specific protein recruitment capacity of four of those site-specific phosphorylations. The proteomics and phosphoproteomics data described in this article is associated with a research article entitled "Characterization of receptor-associated protein complex assembly in Interleukin (IL)-2-and IL-15-activated T-lymphocytes" (Osinalde et al., 2016 [1]

Type of data
Mass Spectrometry (MS) data How data was acquired MS data was acquired in a Q-Exactive (Thermo) mass spectrometer and Velos Orbitrap MS system (Thermo).

Experimental features
For IL-2R interactome and phosphorylation analyses, after stimulation, cells were lysed and protein extracts derived from the three different experimental conditions were combined and affinity-purified using specific antibodies against IL-2R beta or gamma subunits. Immune complexes were fractionated on a SDS-PAGE and in-gel digested using trypsin. Resulting peptides were either directly analyzed by LC-MS/MS or enriched in phosphorylated peptides using TiO 2 beads prior to MS analysis using a QE-Exactive MS instrument.
For peptide pull-down analyses, differentially labeled cells were lysed and separately incubated with the unmodified and phosphorylated version of the peptide used as bait. Then precipitated proteins were on-bead digested with LysC and subsequently with trypsin and resulting peptides were analyzed by LC-MS/MS in a Velos Orbitrap MS system.

Data source location
Odense, Denmark Data accessibility Data are available in this article and deposited at ProteomeEXchange Constorium, http://www.proteomexchange.org/.

Value of the data
The study uncovers new IL-2-and IL-15-dependent interacting partners of IL-2Rβ and IL-2Rγ. This investigation provides unprecedented data regarding cytokine-dependent and -independent phosphorylation events occurring in IL-2R β and γ subunits. A large number of phosphosites corresponding to a wide range of proteins are reported. The data presented here underscores the capacity of certain cytokine-dependent phosphorylation sites localized on IL-2Rβ and IL-2Rγ to recruit downstream signaling molecules.
Overall, the study provides novel insights into the early activation events following interleukin/ receptor engagement in CD4 þ T-lymphocytes.

Data
The data in this article show the effect of IL-2 and IL-15 stimulation on the phosphorylation state and interacting partners of IL-2Rβ and IL-2Rγ. Data on the capacity of selected phosphorylations to serve as anchoring sites and recruit proteins are also presented. In all the experiments a SILAC-based approach was followed in combination with LC-MS/MS and subsequent bioinformatic analyses.
In order to achieve complete labeling of the proteome, Kit225 T-cells were maintained in SILAC media for at least five cells divisions.

Cytokine stimulation and protein extraction
To study the interactome of IL-2Rβ and IL-2Rγ in resting and interleukin-treated T-cells a triple SILAC-based MS strategy was carried out. Prior to the treatment, Kit225 T-cells were deprived with the cytokine for 48 h to synchronize them at G 1 phase of the cell cycle and mimic resting Tlymphocytes. Subsequently, interleukin receptor complex assembly was induced by incubating medium-and heavy-labeled Kit225 T-cells (50 Â 10 6 cells/ml) with 200 U/ml of human recombinant IL-2 or IL-15 (Peprotech) respectively, for 5 min at 37°C in a water bath. T-cells grown in light media were kept untreated and served as control (Fig. 1).
Four different peptide/phosphopeptide pull down assays were carried out. For each of them, a double SILAC experiment was perform in duplicate where Kit225 T-cells were grown either in light or heavy medium. Cells were starved for 2 days and then, the same procedure was applied to both cellular conditions: half of the cell population was kept unstimulated whereas the other half was treated with 200 U/ml IL-2 as mentioned above.
Interleukin stimulation was quenched by rapidly diluting cells on chilled PBS and washing them twice with ice-cold PBS. Differentially treated cells were lysed separately by incubating with rotation at 4°C with co-immunoprecipitation buffer (25 mM TrisHCl pH 7.5, 100 mM NaCl, 1% NP-40, 1 mM sodium pervanadate, 5 mM beta-glycerophosphate, 5 mM NaF, complete protease inhibitor cocktail (complete tablets, Roche)) and lysates were then cleared by centrifugation (15,000g, 15 min at 4°C). Protein concentrations of the soluble fractions were estimated using BCA protein assay kit (Pierce) following manufacturer's instructions.

Immunoprecipitation of IL-2R beta and gamma subunits
Light, medium and heavy labeled protein lysates derived from untreated, IL-2-stimulated and IL-15-stimulated T-cells respectively, were equally combined (in 1:1:1 ratio) according to their protein concentration and incubated with 50% slurry protein A-sepharose (GE Healthcare) for 1 h at 4°C to eliminate proteins that bind non-specifically to the beads. Pre-cleared protein lysates were then incubated with antibodies against IL-2Rβ (sc-671) or IL-2Rγ (sc-667) at 4°C for 4 h with rotation ( Fig. 1). Then, immunoprecipitates were washed five times with lysis buffer and eluted by resuspending in Laemmli buffer and boiling at 95°C for 5 min.

Fractionation and in-gel digestion of immunoprecipitated proteins
Proteins that were immunoprecipitated and hence, eluted were fractionated in two parallel lanes by one dimensional gel electrophoresis using a precast gradient NuPAGE 4-12% Bis-Tris Gel (Invitrogen) and stained with Colloidal Blue (Invitrogen). According to the bands visualized, the gel was cut into different size slices that were subsequently treated, as previously described in detail (our data in brief), with reducing and alkylating agents followed by trypsin so that the proteins undergo digestion. Resulting peptides were extracted from the gel by serial incubations with 100% acetonitrile (ACN) and 30% ACN/ 3% TFA at room temperature. Finally, recovered peptides were concentrated using a vacuum centrifuge before MS analysis (Fig. 1).

Enrichment of phosphorylated peptides using TiO 2 beads
Enrichment of phosphopeptides was performed using titansphere beads [4] and following the protocol previously described in detail [4].

Peptide pull-down analysis
A series of N-terminally biotinylated phosphopeptide/peptide pairs were purchased from Pepti-de&Elephant: IL-2Rβ pS431/S431, SGDDLLLFSPSLLGG; IL-2Rβ pT476/T476, SGQPLGPPTPGVPDL; IL-2Rγ pY325/Y325 SGLAESLQPDYSERLCL and IL-2Rγ pY357/Y357 SGPCNQHSPYWAPPCY. The phosphorylated residue is highlighted in bold and underlined. Two pull down assays were carried out using each pair of peptides. In one of the replicas, protein extracts derived from T cells grown in light media were incubated with the unmodified peptide whereas proteins extracted from T cells grown in heavy media were incubated with the corresponding phosphorylated version of the same peptide. By contrast, in the second replica the labels were swapped so that heavy labeled proteins were incubated with the unmodified peptide and light proteins with the phosphopeptide (Fig. 2). It should be mentioned that in all the experiments, half of the T-cell population grown either in light or heavy media was kept unstimulated whereas the remaining half was treated with IL-2 for 5 min. Proteins were extracted by incubation in the co-immunoprecipitation buffer as described before (Section 2.2) and same amounts of differentially treated light and heavy protein extracts were combined. Subsequently, to remove sticky proteins from the samples light and heavy proteins were separately incubated with Dynabeads MyOne Streptavidine C1 (Thermo) for 2 h at 4°C with rotation.
Meanwhile biotinylated peptides were coupled to Dynabeads MyOne Streptavidine C1 (Thermo) by incubating for at least 2 h at 4°C in rotation. Unbound peptides were removed and pre-cleared protein lysates were added to the corresponding peptide or phosphopeptide and incubated for 2.5 h with rotation at 4°C. Then, beads were subjected to 3 washes with complete lysis buffer and additional 3 washes with lysis buffer without detergent. Finally, beads loaded with the distinct version of the same peptide were combined.

On-bead digestion
Proteins precipitated with the phosphorylated or corresponding unmodified peptide were simultaneously subjected to on-bead digestion. For that purpose streptavidin beads loaded with the peptides and attached proteins were dissolved in urea buffer (8 M urea, 10 mM TrisHCl pH 7.5). Proteins were subjected to reduction and alkylation by 1 h incubation at room temperature using 1 mM DTT and 5.5 mM iodoacetamide, respectively. Then, Lys-C endoproteinase was added and the lysates were digested for 4 h at 37°C. The resulting peptide mixtures were diluted with 10 mM TrisHCl pH 7.5 to achieve a final urea concentration below 2M prior to addition of trypsin that was incubated with the lysates at 37°C over night. Trypsin activity was quenched by acidification using trifluoroacetic acid (TFA) to a final concentration of 1%. Prior to MS analysis, peptides were desalted and fractionated with C 18 stage-tips by eluting with increasing acetonitrile concentration.

Mass spectrometry analysis
All procedures are described in detail in the associated research article [1].

Data analysis
MaxQuant software version 1.3.0.5 [5] was used to process the.raw files generated applying the parameters described before [6]. For protein identification, a minimum of 2 peptides, including 1 unique was required. Cysteine carbamidomethylation was set as fixed modification whereas M oxidation, N-terminal acetylation, NQ deamidation and STY phosphorylation were set as variable modifications.
Supplementary Tables 1 and 2 contain information about all identified and quantified proteins in the IL-2Rβ and IL-2Rγ immunoprecipitation experiments. Proteins displaying a cytokine (IL-2 or IL-15)/control 4 2 and Significance B p value o0.001 were considered as cytokine-dependent binding partners of IL-2Rβ and/or IL-2Rγ. We used the STRING database [7] to evaluate the protein-protein   4. Site specific phosphorylations on IL-2R beta and gamma subunits. Phosphosites highlighted in red were found to be induced upon cytokine stimulation (cytokine/control ratio 4 2) whereas the ones in black were not affected by the treatment. No SILAC ratio could be recorded for the pS538-containing peptide. *indicates that the phosphosite is not reported in the PhosphositePlus database.
interactions that were already reported within our list of cytokine-induced interactors of IL-2Rβ and IL-2Rγ (Fig. 3). As mentioned above part of the immune complexes containing IL-2Rβ and IL-2Rγ were subjected to trypsin digestion and TiO 2 -based enrichment of phosphopeptides prior to MS analysis. For the analysis of phosphopeptides a minimum localization probability of 0.75 was required. A total of 6 and 4 site specific phosphorylations corresponding to IL-2Rβ and IL-2Rγ respectively were detected ( Fig. 4 Table 3). Moreover, Supplementary Table 4 contains information regarding additional phosphosites detected in this study.

and Supplementary
Supplementary Tables 5-8 contain information regarding all the proteins quantified in the two replicates of the 4 distinct peptide pull down assays performed. Proteins displaying a phosphopeptide/peptide ratio 4 2 were considered as phosphosite-dependent binding partners of IL-2Rβ and/or IL-2Rγ. We used the STRING database [7] to evaluate the protein-protein interactions that were already reported within our list of phosphosite-dependent interactors of IL-2Rβ and IL-2Rγ (Fig. 5).