Dataset of proteomics analysis of aging C. elegans exposed to Pseudomonas aeruginosa strain PA01

Here, we present the proteomics dataset of young and middle-aged Caenorhabditis elegans (C. elegans) exposed to Pseudomonas aeruginosa (P. aeruginosa strain PA01), which is related to the article "Proteomic Identification of Virulence-Related Factors in Young and Aging C. elegans infected with Pseudomonas aeruginosa" (C. D. King et. al, in-revisions). This dataset was generated to better understand the effects of aging on molecular mechanisms involved in host response to pathogen exposure. Protein from C. elegans of different age and exposure to P. aeruginosa PA01 or control E. coli OP50 were extracted and tryptically digested. Peptides were labeled with the reagents tandem mass tag (TMT6-plex), separated, and detected by using offline strong-cation exchange and online liquid chromatography – mass spectrometry (SCX – LC – MS/MS & MS3). A separate mixture of peptides were labeled on N-terminal amines and lysines with dimethylation. Dimethylated peptides were analyzed using LC – MS/MS and a portion of the results were used to verify fold-change direction for TMT6-plex experiments. Raw data can be found online at www.CHORUSproject.org, a cloud-based data repository (see specifications table for details).


Specifications
Raw mass spectra, un-filtered PD read-out files, and analyzed PD excel files Experimental factors Young (Day 1) and middle-aged (Day 5) C. elegans exposed to P. aeruginosa strain PA01 and aged-matched controls were used. C. elegans were grown to adulthood on E. coli OP50, then half were transferred to P. aeruginosa PA01 and exposed for 18 h. After pathogen exposure, C. elegans were harvested and homogenized, yielding protein homogenates for proteomics analysis. Experimental features Young (Day 1) and middle-aged (Day 5) C. elegans exposed to P. aeruginosa PA01, aged-matched controls, and a pooled sample containing equal amounts of the four samples were analyzed using proteomics techniques. Protein samples were digested with trypsin and resulting peptides were labeled by either TMT 6 tags or dimethylation. Mass spectra and resulting Excel files provide both qualitative and quantitative information about the aging proteome of C. elegans exposed to P. aeruginosa PA01.
This data may be useful for individuals interested in comparing the proteome of C. elegans exposed to P. aeruginosa PA01 in comparison to various pathogens.
This data extends the information available for proteome changes in aged-adult C. elegans. Data relating to changes in metabolism in middle-aged C. elegans exposed to P. aeruginosa PA01 may be used to gain further insight about the innate response of aging in higher-ordered organisms.

Data
Data presented here was generated by analyzing young and middle-aged C. elegans exposed to P. aeruginosa PA01, aged-matched controls, and a pooled sample containing equimolar amounts of all the samples. This dataset includes RAW mass spectra, Proteome Discoverer (PD) read-out files (Excel), and statistical analysis (Excel). RAW files generated were processed using PD, read-outs from the protein software were subjected to filtering and the Power Analysis statistical method [1], and final protein lists were searched using data analysis platforms (i.e PANTHERdb, Uniprot, and Wormbase).  Table 1 explains the chemical-labels used for both sets of experiments.

Sample preparation for proteomics analysis
Sodium hypochlorite L1synchronized worm populations were obtained and reared to early adulthood on E. coli OP50seeded NGM at 20°C, ( $ 80,000-100,000 total worms). To examine the proteome of young adults challenged with P. aeruginosa, one group of $ 20,000 worms was transferred to P. aeruginosa PA01seeded SK plates on Day 1 of adulthood for 18 h after transfer to fresh E. coli OP50seeded NGM at 20°C (again, transferring to fresh plates every other day to avoid progeny contamination). The second group was on E. coli OP50seeded NGM plates until Day 5 of adulthood before 18 h pathogen exposure and harvesting of both control and experimental groups. The parents were separated from eggs and progeny during transfers, and for harvesting, adults were washed off Sample preparation workflow of young and middle-aged C. elegans exposed to P. aeruginosa PA01 and aged-matched controls. C. elegans were exposed to P. aeruginosa PA01 for 18 h at either first (young) or fifth (middle-age) day of adulthood. Aged-matched controls were fed on E. coli OP50. After 18 h, worms were harvested, homogenized, and protein was extracted. A pooled sample containing equimolar amounts of the four groups was added. Protein was digested using trypsin and resulting peptides were chemically-labeled using either tandem mass tags (TMT 6 -plex) for the main analysis or dimethylation for the verification analysis. Labeled peptides were pooled, analyzed using LC -MS, and then analyzed using Proteome Discoverer v. 1.4. Fig. 2. Data analysis workflow of aging C. elegans exposed to P. aeruginosa PA01. Tandem MS (MS/MS) and MS 3 spectra were used to generate lists (per technical replicate) of identified and quantified proteins. Statistical analysis was applied to these lists. Proteins with a fold change Z 1.4 or r 0.72 were determined to be statistically significant and added to a final list of differentially-expressed proteins. the plates with M9 and then allowed to settle by gravity. Two independent biological replicates were collected for both age groups to ensure reproducibility of important findings. At their respective time points, worms were floated off plates with M9 buffer [2] and washed two times to remove extraneous bacteria and obtain sample pellets.

Protein extraction
Worms were harvested, washed with M9 buffer to remove bacteria, and centrifuged to obtain a pellet. Pellets were re-suspended in Reassembly (RAB) buffer (0.1 M MES, 1 mM EGTA, 0.1 mM EDTA, 0.5 mM MgSO 4 , 0.75 M NaCl, 0.02 M NaF) with Roche Complete Protease Inhibitor (Roche Applied Science). Protein homogenate was sonicated for 10 s, 50 s on ice, incubated for 10 min on ice and centrifuged at 14000 g. The amount of protein was determined by BCA assay (Thermo Scientific). A pooled sample (Sample #5) containing equimolar ratios of Day 1 E. coli OP50 (Sample #1) and P. aeruginosa PA01 (Sample #2) and Day 5 E. coli OP50 (Sample #3) and P. aeruginosa PA01 (Sample #4) samples, was also prepared.

Protein digestion
Protein was purified using acetone precipitation and the amount of protein was re-determined with BCA assay. Protein ( $ 80-100 mg) was denatured with an extraction buffer (0.

TMT labeling
Digested samples were desalted with an HLB cartridge and dried by centrifugal evaporation. Each sample was labeled with a TMT 6 -plex reagent following the manufacturer's protocol (Thermo Scientific). TMT 6 reagents were equilibrated to room temperature, solubilized with 41 mL of acetonitrile, and transferred to peptide samples reconstituted in triethylammonium bicarbonate (TEAB) buffer. After 1 h of incubation ( $25°C), the reaction was quenched using 5% hydroxylamine. Equimolar amounts of samples were combined such that reagents that generate reporter ions at m/z 126:127:128:129:130 correspond to D1 OP50, D1 PA01, D5 OP50, D5 PA01, and the pooled sample, respectively.

LC-MS analyses
Online desalting and reversed-phase chromatography was performed with a Nano liquid chromatography (LC) system equipped with an autosampler (Eksigent). Mobile phases A and B used for reversed phase (RP)-LC separation of peptides were 3% (v/v) acetonitrile with 0.1% formic acid and 100% acetonitrile with 0.1% formic acid, respectively. SCX fractions (10 μL) were loaded onto a trapping column (100 mm i.d. x 2 cm), which was packed in house with C 18 200 Å stationary phase material (Michrom Bioresource Inc,) at 3 μL/min in 3% mobile phase B for 3 min. After desalting, the sample was loaded onto an analytical column (75 mm i.d. x 13.2 cm) which was packed in-house with C 18 100 Å 3 mm stationary phase material (Michrom Bioresource Inc). The gradient was as follows: 0-7 min, 10% mobile phase B; 7-67 min, 10-30% B; 67-75 min, 30-60% B; 75-77 min, 60-90% B; 77-82 min, 90% B; 82-83 min, 90-10% B; 83-95 min, 10% B. The LC eluent was analyzed with positive mode nanoflow electrospray using a LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). Data-dependent acquisition parameters were as follows: the MS survey scan in the Orbitrap (300-1800 m/z) was 60,000 resolution; the top seven most intense peaks were isolated and fragmented with collision-induced dissociation (CID) in the LTQ (normalized collision energy of 35%). Directly after each tandem MS/MS scan, the most intense fragment ion over the m/z range 200-1545 was selected for higher-energy collisional dissociation (HCD) MS 3 . The fragment isolation width was set to 4 m/z, the MS 3 AGC was 3 Â 10 5 , the normalized collision energy was 60%, the resolution was 7,500 and the maximum ion time was 250 ms. HCD spectra were recorded in the Orbitrap. Each fraction was subject to duplicate injections.

Data analyses
RAW files were analyzed with Proteome Discoverer 1.4 software (Thermo Scientific) and searched against the Uniprot C. elegans database (11/26/2013, 25,673 sequences). SEQUEST search parameters were as follows: two maximum trypsin mis-cleavages, precursor mass tolerance of 10 ppm, fragment mass tolerance of 0.8 Da; static modifications were TMT six-plex/ þ 229.163 Da (N-terminus, Lys) and carbamidomethyl modification/ þ 57.021 Da (Cys); dynamic modification was oxidation modification/ þ15.995 Da (Met). Decoy database searching was employed to generate medium (p o0.05) and high (p o0.01) confidence peptide lists. All the peptides with medium and high confidence were used to identify and quantify proteins. To filter peptides, the following parameters were applied: peptide confidence level of medium or high, peptide rank of 1, and peptide deviation of 10 ppm. Peptides with a PSM (peptide to spectral match) count of 1 (per technical replicate) were not considered for analysis. The reporter ions (i.e. m/z 126-130) were identified with the following parameters: most confident centroid and 20 ppm for reporter ion mass tolerance. Furthermore, reporter ion values were normalized 126/130, 127/130, 128/130, and 129/130 and final ratio reporting given as 127/126 and 129/128. Proteins belonging to multiple isoforms were grouped into a single accession number and final ratios were reported.

Statistics
Coefficient of variation (CV) values were calculated as previously explained for reporter ion ratios of proteins quantified in both biological replicates [1]. The mean CV value from both biological replicates was calculated and used as the total biological variation, S b , (i.e. 0.36). The technical variation, S t , was calculated for proteins quantified in at least one technical replicate of each biological replicate, and was 0.22. This power analysis was used to generate an appropriate fold-change cutoff for TMT 6 -plex data. Filter criteria were applied to generate a list of statistically significant differentially-expressed proteins as follows: 1) proteins identified and quantified in at least one technical replicate (per biological replicate), 2) CV values r 0.36, and 3) fold-change cutoff dependent upon n as such Z1.4 or r0.72 (n ¼2).
Data from the dimethylation experiment was treated by applying the following criteria: 1) proteins identified and quantified in both technical replicates and 2) fold-change cutoff of Z 1.4 or r0.70 dependent on p o0.05.