Data describing the association between rs266729 polymorphism inadiponectin promoter gene and Type 2 Diabetes Mellitus

This article investigates whether there is an association between the rs266729 polymorphism in adiponectin promoter gene with metabolic parameters and disease status in 300 type 2 diabetes patients and 300 healthy adults from Jahrom city, Iran. The variants (G/C) were tested by polymerase chain reaction-restriction fragment length polymorphism method (RFLP) and metabolic parameters (glucose, cholesterol, HDL and LDL cholesterol) were measured using biochemical methods. However, no differences were detected between the haplotypes investigated, and the data obtained from our lab shown association of the ADIPOQ promoter polymorphism neither with biochemical parameters, nor with disease status.


a b s t r a c t
This article investigates whether there is an association between the rs266729 polymorphism in adiponectin promoter gene with metabolic parameters and disease status in 300 type 2 diabetes patients and 300 healthy adults from Jahrom city, Iran. The variants (G/C) were tested by polymerase chain reaction-restriction fragment length polymorphism method (RFLP) and metabolic parameters (glucose, cholesterol, HDL and LDL cholesterol) were measured using biochemical methods. However, no differences were detected between the haplotypes investigated, and the data obtained from our lab shown association of the ADIPOQ promoter polymorphism neither with biochemical parameters, nor with disease status. & 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Biology More specific subject area

Endocrinology, diabetes
Type of data

Experimental features
Polymorphism rs266729 of ADIPOQ gene was tested by RFLP using HhaI enzyme and metabolic parameters were measured using biochemical methods in type 2 diabetic patients and healthy adults Data source location Jahrom university, Iran Data accessibility Data supplied with this article

Value of the data
We evaluated the association one of the adiponectin gene polymorphisms with diabetes type 2. This data is useful for characterising the relationship between heritable traits and the polymorphism.
Our data can provide insight into methodology and considerations for investigation of polymorphisms in non-coding regions.

Data
We investigated the potential association between the ADIPOQ gene polymorphism and metabolic parameters in type 2 diabetic patients. The clinical data and baseline characteristics of the participants are shown in Table 1. The frequency distribution of genotypes (G/C) rs266729 polymorphism in healthy subjects and patients is given in Table 2. As GG mutant genotype frequencies between the two groups of diabetic patients and healthy, as well as the frequency of the G allele showed no significant difference between the groups (P Z0.05). The relationship between the compositions of the CC genotype against GC þGG with biochemical factors in both patients and controls were evaluated. As shown in Table 3. No significant relationship was observed between biochemical factors and this polymorphism (PZ 0.05).

Characteristics of patients
A cross sectional study was conducted and samples were determined using statistical calculations. All participants were in the Fars area and Composition of the population in this area is rare. This research conforms to the declaration of Helsinki and approved by the ethics research committee of Jahrom University of Medical Sciences. Each participants had consent to partake in the study and, based on the testimonial, they could leave the study. This study was approved by the Research Ethics Committee of Jahrom University of Medical Sciences.

Genotyping
3-5 ml of venous blood was taken from each of the subjects after 12 h of fasting. DNA was extracted from nucleated blood cells, according to the DNA extraction protocol kit (cinagenne Co., Iran).