Morbility, clinical data and proteomic analysis of IUGR and AGA newborns at different gestational ages

The data are related to the proteomic analysis of 43 newborns with intrauterine growth retardation (IUGR) and 45 newborns with appropriate weight for gestational age (AGA) carried out by separation via 2DE and analyzed by MS–TOF/TOF. All newborns were separated into three gestational age groups, "Very Preterm" 29–32 weeks, "Moderate Preterm" 33–36 weeks, and, "Term" ≥37weeks. From each newborn, blood was drawn three times from birth to 1 month life. High-abundant serum proteins were depleted, and the minority ones were separated by 2DE and analyzed for significant expression differences. The data reflect analytic and clinic variables analyzed globally and categorized by gestational age in relation to IUGR and the optimization of conditions for 2-DE separation. The data from this study are related to the research article entitled "Alterations of Protein Expression in Serum of Infants with Intrauterine Growth Restriction and Different Gestational Ages" (M.D. Ruis-González, M.D. Cañete, J.L. Gómez-Chaparro, N. Abril, R. Cañete, J. López-Barea, 2015) [1]. The present dataset of serum IUGR newborn proteome can be used as a reference for any study involving intrauterine growth restriction during the first month of life.


a b s t r a c t
The data are related to the proteomic analysis of 43 newborns with intrauterine growth retardation (IUGR) and 45 newborns with appropriate weight for gestational age (AGA) carried out by separation via 2DE and analyzed by MS-TOF/TOF. All newborns were separated into three gestational age groups, "Very Preterm" 29-32 weeks, "Moderate Preterm" 33-36 weeks, and, "Term" Z37weeks. From each newborn, blood was drawn three times from birth to 1 month life. High-abundant serum proteins were depleted, and the minority ones were separated by 2DE and analyzed for significant expression differences. The data reflect analytic and clinic variables analyzed globally and categorized by gestational age in relation to IUGR and the optimization of conditions for 2-DE separation. The data from this study are related to the research article entitled "Alterations of Protein Expression in Serum of Infants with Intrauterine Growth Restriction and  The  present dataset of serum IUGR newborn proteome can be used as a  reference for any study involving intrauterine growth  Data accessibility The data are supplied with this article

Value of the data
The clinical characteristics of IUGR neonates, and the statistical analysis of quantitative and qualitative variables, carried out both globally and in function of gestational age can be useful for other scientists working in the IUGR field.
The set-up and optimization of the conditions of 2-DE analysis in sera from newborns could be useful for the reproducibility of similar future trials.
This dataset could be used as a benchmark for studies on the variations of serum proteomic IUGR from birth through the first month of life.
For the development of 2-DE electrophoresis, we started carrying our 2-DE gels with a wide pH range (3-10), as shown in Fig. 1A. In these initial gels we observed that most of the proteins were in the pH range 4-7. Thus, we reasoned that the resolution would improve using IPG strips of narrower pH gradient. Thus, 2-DE electrophoresis was routinely carried out using IPG strips of 11 cm and pH range 4-7, as shown in Fig. 1B. Isoelectric focusing was performed using an Protean IEF Cell (BioRad) a 20°C according the next program (Table 8).

Subject selection
We included IUGR neonates, defined by having a birth weight o10th centile for gestational age (GA), according to Carrascosa curves [2], together with echographic evidence of altered placental function, identified as an abnormal Doppler of the umbilical artery, or reduced growth rate [3]. Also

Statistical analysis
The descriptive analysis of the variables studied was carried out with the routine methodology. Comparison of variables between the RCIU and AGA groups was carried out using the Student t-test for quantitative variables and the χ 2 -test for qualitative variables. The Fisher exact test was applied when conditions of applicability of the χ 2 -test were not fulfilled. A value of po0.05 was established as statistically significant. The R statistical software, version 3.22, was used throughout the whole study [4].