Dataset of differentially regulated proteins in HUVECs challenged with wild type and UGM1 mutant Aspergillus fumigatus strains

Invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen Aspergillus fumigatus. The molecular mechanisms associated with A. fumigatus infection in the vascular endothelium are poorly understood. In this context, we used a high-throughput proteomic approach to unveil the proteins modulated in HUVECs after interaction with a wild type strain and the UGM1 mutant (Δugm1) of A. fumigatus. The proteomic analysis was also performed in HUVECs challenged with a galactosaminogalactan (GAG) purified from A. fumigatus cell wall. The dataset presented here correspond to all proteins identified that fit a 2-fold change criteria (log 2 ratio ≥ 1 or ≤ −1), disregarding the statistical validation cut off, in order to supplement the research article entitled “Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates the HUVEC proteins associated with inflammatory and stress responses” (G.W.P. Neves, N.A. Curty, P.H. Kubitschek-Barreira, T. Fontaine, G.H.M.F. Souza, M. Lyra Cunha, G.H. Goldman, A. Beauvais, J.P. Latgé, L.M. Lopes-Bezerra, 2016) [1]. The mass spectrometry proteomic data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002823.

Aspergillus fumigatus HUVEC GAG UGM1 mutant PROTEOME Invasive aspergillosis a b s t r a c t Invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen Aspergillus fumigatus. The molecular mechanisms associated with A. fumigatus infection in the vascular endothelium are poorly understood. In this context, we used a high-throughput proteomic approach to unveil the proteins modulated in HUVECs after interaction with a wild type strain and the UGM1 mutant (Δugm1) of A. fumigatus. The proteomic analysis was also performed in HUVECs challenged with a galactosaminogalactan (GAG) purified from A. fumigatus cell wall. The dataset presented here correspond to all proteins identified that fit a 2-fold change criteria (log 2 ratio Z 1 or r À1), disregarding the statistical validation cut off, in order to supplement the research article entitled "Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates the HUVEC proteins

Subject area
Biology More specific subject area

Medical Mycology
Type of data All HUVEC protein extracts were analyzed using a 2D-nanoLC-HDMS E approach.

Rio de Janeiro, Brazil
Data accessibility Data is within this article and deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002823.

Value of the data
This dataset will be of value to the scientific community aiming to analyze the identified proteins modulated in HUVECs upon infection by A. fumigatus and the participation of the cell wall galactosaminogalactan (GAG) in endothelial activation.
This data article contributes to the scientific research concerning the role of GAG in the physiopathology of invasive aspergillosis.
The data provided here identify key proteins involved in the response of HUVECs to different strains of A. fumigatus, contributing to the knowledge of angioinvasive molecular mechanism in invasive aspergillosis.
For scanning electron microscopy (SEM), the conidia were placed on glass coverslips coated with 2% gelatin and incubated in Sabouraud broth for 6 h at 37°C, to obtain the germ tubes (germlings). After, germlings were washed in PBS and fixed for 1 h with 2.5% glutaraldehyde plus 4% paraformaldehyde in 0.1 M calcodylate buffer (pH 7.2), washed three times with the same buffer and postfixed with 1% osmium tetroxide. The cells were subsequently dehydrated in a graded ethanol series  and critical point-dried with CO 2 . The specimens were coated with gold in a Balzers sputtering apparatus and the micrographs were captured using a JEOL JSM-6510 LV SEM. The images were processed using ImageJ software. The obtained scanning electron microscopy (SEM) images of germlings of different A. fumigatus strains are shown in Fig. 1A. This experiment was performed twice with experimental duplicates. For GAG immunolabeling, the conidia were germinated overnight in Brian's medium [6] on eightwell Permanox chamber slides (Lab-Tek s ) at 30°C and further fixed with 2.5% (w/v) paraformaldehyde for 2 h at room temperature. After fixation, the cells were washed with 0.1 M NH 4 Cl in PBS for 5 min, followed by incubation with 5% goat serum in PBS for 1 h. The cells were incubated with an anti-galactosaminogalactan monoclonal antibody (kindly provided from Dr. Jean-Paul Latgé, Pasteur Institute, Paris) at 20 mg/ml in 5% goat serum/PBS for 1 h at room temperature. After washing with goat serum/PBS, the cells were incubated with goat TRITC-conjugated anti-mouse IgG(H þL) (Sigma) diluted 1:200 in goat serum/PBS. After washing with PBS, hyphae of A. fumigatus strains were visualized with a fluorescence light microscope, and the images are shown in Fig. 1B. A mock monoclonal antibody was used as a control. This experiment was performed three times with experimental duplicates.

Culture of human vein endothelial cells (HUVEC) and interaction assay
HUVECs were obtained as previously described in [1,7]. The use of HUVECs was approved through the Research Ethics Committee of the Municipal Health Secretary and Civil Defense of Rio de Janeiro (CEP SMSDC-RJ), protocol no. 196/09. In total, 18 volunteers participated in the present study.
For the interaction assays, HUVEC monolayers were infected with germlings of A. fumigatus WT and Δugm1 strains, and with purified GAG (1 mg/mL), as described previously [1]. Uninfected HUVEC monolayers (control) were maintained in the same culture conditions. For SEM the HUVEC assays were performed as described above, section 1.1. The SEM images of HUVEC monolayers challenged with germlings of all A. fumigatus strains are shown in Fig. 2.

Label-free protein analysis using mass spectrometry, database searching and quantification
The proteins extracts used for proteomics assays were obtained as follows. The endothelial cell monolayers cultivated on 75-cm 2 culture flasks were infected with thimerosal-killed germlings of A. fumigatus [4] or were interacted with the purified GAG. After, HUVEC monolayers were washed twice with Hank´s balanced salt solution (Cultilab) and gently harvested using a cell scraper. Next, the cells were centrifuged at 200 g, for 10 min. The pellet was subsequently suspended in 250 mL of a lysis buffer (8 M urea, 1 M Tris, 4% (w/v) CHAPS, supplemented with 1 mM PMSF, 5 mM EDTA, 160 mM leupeptin, 1 mM pepstatin, and 0.125 units/ml benzonase) and incubated for 1 h at 4°C. Subsequently, the cell lysate was centrifuged at 13,000 g, for 15 min at 4°C, and the supernatants (protein extracts) were collected and stored at À80°C. After extraction, the proteins were quantified using a Bradford assay and analyzed as previously described [8]. Briefly, 50 mg of each extract was concentrated, and the lysis buffer was exchanged with 50 mM ammonium bicarbonate using a 3-kDa ultra-filtration device (Millipore). The protein extract was denatured (0.1% RapiGEST SF at 60°C for 15 min) (Waters, Milford, USA), reduced using DTT (10 mM DTT at 60°C for 30 min), alkylated with IAA (10 mM IAA at room temperature for 30 min in the dark) and enzymatically digested with trypsin (Promega, Madison, USA) at a 1:50 (w/w) enzyme:protein ratio. This reaction was terminated after the addition of 10 mL of 5% trifluoroacetic acid (TFA), and the internal standard, yeast alcohol dehydrogenase (P00330), was added to the digests to achieve 10 fmol μL À 1 per injection [9,10]. Qualitative and quantitative bi-dimensional nanoUPLC tandem nanoESI-HDMS E experiments were conducted using a 1 h reverse-phase gradient from 7% to 40% (v/v) acetonitrile (0.1% v/v formic acid) at 500 nL min À 1 on a nanoACQUITY UPLC 2D Technology system. A nanoACQUITY UPLC HSS T3 1.8 mm, 75 mm Â 15 cm column (pH 3) was used in conjunction with a reversed phase (RP) XBridge BEH130 C18 5 mm 300 mm x 50 mm nanoflow column (pH 10). A typical on-column sample load was 500 ng of total protein digests for each of the five fractions (500 ng/fraction/load). The resolving power for all measurements was at least 35,000 FWHM, and the ion mobility cell, filled with nitrogen gas, had a cross-section resolving power of at least 40 Ω/ΔΩ. The effective resolution with the conjoined ion mobility was 41,800,000 FWHM. The samples were ionized using a NanoLockSpray source (Waters, Manchester, UK) in the positive ion mode of nanoESI ( þ). The lock mass channel was sampled every 30 s.
The mass spectrometer was calibrated with MS/MS spectrum of Glu-Fib peptide solution (100 fmol mL À 1 ) delivered through the reference sprayer of the NanoLockSpray source. The doubly charged molecule [M þ 2H] 2 þ ¼ 785.8426 was used for initial single-point calibration and MS/MS fragment ions of Glu-Fib, such as a þ , b þ , and y þ , were used to obtain the final instrument calibration. A Synapt G2-S HDMS instrument (Waters, Manchester, UK) was tuned to perform HDMS E multiplexed DIA acquisition scanning with added specificity, orthogonality and selectivity and low and high collision energies in a T-wave transfer cell [11]. The mass spectrometer was automatically set to (a) switch between low energy MS (4 eV) and elevated collision energies HDMS E (19-45 eV) applied to the transfer "T-wave" collision-induced dissociation (CID) cell filled with argon gas; and (b) adjust the trap collision cell to 1 eV for the total ion current. The scan time was previously adjusted based on the linear velocity of the chromatography peak delivered to the ion source to obtain a minimum of 20 scan points for each single peak, under both low-energy and high-energy transmission at an orthogonal acceleration time-of-flight (oa-TOF) and a range of 50-2000 m/z. The RF offset (MS profile) was adjusted, such that the nanoUPLC-HDMS E data were effectively transmitted from 400 to 2000 m/z, ensuring that any masses observed in the high-energy spectra with less than 400 m/z arose from dissociations in the collision cell.
Protein identification and quantitative data packaging were performed as previously described [1,[12][13][14][15]. A cut-off Log2ratio values of þ1.0 (2-fold) or higher was applied for determining the proteins with higher abundance levels and À 1.0 (2-fold) or lower for proteins with lower abundance levels to compare pairs of experimental groups [16,17]. In silico prediction of protein-protein interaction and pathways of HUVEC proteins, with different abundance levels after interaction, was performed using QIAGEN's Ingenuity s Pathway Analysis (IPA s , QIAGEN Redwood City, www.qiagen.com/ ingenuity) and DAVID Bioinformatics Resources 6.7. The proteomic data were deposited in the Pro-teomeXchange Consortium [18] via the PRIDE [19] partner repository with the dataset identifier PRIDE: PXD002823.
The list of proteins with different abundance ratio filtered with 2-fold change criteria in Δugm1 vs. control, WT vs. control; Δugm1 vs. WT; and GAG vs. control are presented in Tables 1-3. Pathways related to Δugm1 (Δugm1 vs. control and Δugm1 vs. WT) interaction conditions are shown in Fig. 3. Furthermore, coincident pathways between Δugm1 and GAG interaction conditions are shown in Fig. 4. Fig. 5 exemplifies one of these coincident pathways (ILK signaling).