Data on nitric oxide production by human bone marrow-derived mesenchymal stromal cells

Due to its anti-inflammatory and immunosuppressive potential, Nitric oxide (NO), a gaseous radical, is of special importance during graft-versus-host diseases (GVHD) and feoto-maternal tolerance. NO is a major mediator of murine mesenchymal stromal cells (MSCs)-immunosuppressive capacity. In this data article, we characterized NO production by human bone marrow-derived MSCs (hBMSCs). MSCs, isolated from healthy donors (n=5), were defined according to the International Society for cellular Therapy (ISCT) guidelines. Based on a fluorometric detection system, and upon using Nitrite (NO2−)/Nitrate ( NO3−) Assay Kit, the amounts of NO metabolites ( NO2− and NO3−) produced by hBMSCs, being grown in a culture medium either lacking (constitutive condition) or containing IL-4, IL-10 or a pro-inflammatory cytokine cocktail made of IL-1β, TNF-α, IFN-α and IFN-γ, were assessed. All assays were carried out in triplicates and the mean values are reported. The data from this study supports and corroborates the discussion associated with our previously published work entitled “The Immunomodulatory Potential of Mesenchymal Stromal Cells: A Story of a Regulatory Network” (Najar et al., 2016) [1].


Potential of Mesenchymal Stromal Cells: A Story of a Regulatory
Network" (Najar et al., 2016) [1]. & 2016 Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Biology More specific subject area

Mesenchymal stromal cells (MSCs)
Type of data Figure  How

Value of the data
This data provides evidences that NO is not constitutively produced by hBMSCs This data will be beneficial for the scientific community focusing on understanding the molecular mechanisms by which hBMSCs modulate the function of their target cells This data could help to understand the varied therapeutic efficiency exhibited by MSCs-derived from different organisms.

NO detection
After culturing hBMSCs under the different conditions (basic versus cytokine primed), their supernatants were collected by centrifugation where Nitrite (NO À 2 ) and Nitrate (NO À 3 ) concentrations were determined using the Nitrite/Nitrate Assay Kit (Sigma). Using this assay, fluorometric detection of Nitrite (NO À 2 ) concentration, in a sample, was achieved upon allowing a chemical reaction between Nitrite (NO À 2 ) and 2,3-diaminonaphthalene (DAN) to generate a fluorescent product called naphthotriazole. The fluorescence intensity was read using a fluorescence reader (

Statistical analysis
The data were analyzed with Optima software (BMG Labtech, Ortenberg, Germany). Three separate experiments were carried out in triplicate and averaged. Data are presented as means 7 SD and analyzed using Wilcoxon Signed Rank test. P-Values o0.05 were considered significant.