Dataset of Arabidopsis plants that overexpress FT driven by a meristem-specific KNAT1 promoter

In this dataset we integrated figures comparing leaf number and rosette diameter in three Arabidopsis FT overexpressor lines (AtFTOE) driven by KNAT1 promoter, “A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis” [5], vs Wild Type (WT) Arabidopsis plats. Also, presented in the tables are some transcriptomic data obtained by RNA-seq Illumina HiSeq from rosette leaves of Arabidopsis plants of AtFTOE 2.1 line vs WT with accession numbers SRR2094583 and SRR2094587 for AtFTOE replicates 1–3 and AtWT for control replicates 1–2 respectively. Raw data of paired-end sequences are located in the public repository of the National Center for Biotechnology Information of the National Library of Medicine, National Institutes of Health, United States of America, Bethesda, MD, USA as Sequence Read Archive (SRA). Performed analyses of differential expression genes are visualized by Mapman and presented in figures. “Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering” [2], described the interpretation and discussion of the obtained data.


a b s t r a c t
In this dataset we integrated figures comparing leaf number and rosette diameter in three Arabidopsis FT overexpressor lines (AtF-TOE) driven by KNAT1 promoter, "A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis" [5], vs Wild Type (WT) Arabidopsis plats. Also, presented in the tables are some transcriptomic data obtained by RNA-seq Illumina HiSeq from rosette leaves of Arabidopsis plants of AtF-TOE 2.1 line vs WT with accession numbers SRR2094583 and SRR2094587 for AtFTOE replicates 1-3 and AtWT for control replicates 1-2 respectively. Raw data of paired-end sequences are located in the public repository of the National Center for Biotechnology Information of the National Library of Medicine, National Institutes of Health, United States of America, Bethesda, MD, USA as Sequence Read Archive (SRA). Performed analyses of differential expression genes are visualized by Mapman and presented in figures. "Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering" [2], described the interpretation and discussion of the obtained data.
& Value of the data The amplification of transgene by PCR and copy variation number by ddPCR is fundamental to compare independent transgenic events.
ANOVA and T-Student test were employed to assess statistical significance of data (α ¼ 0.05) for leaf count and rosette diameter in order to compare three AtFTOE lines and WT.
Differentially expressed genes in the context of cellular functions are graphically presented by Mapman to understand the integrative changes in the metabolism.
Raw data from the Illumina HiSeq sequencing are available for further analyses.

Data
In this article are presented the data analyses (figures) from leaf count and rosette diameter for three lines AtFTOE (2.1, 3.1 an 4.3) compared with WT Arabidopsis plants ( Fig. 2A and B respectively). Data corresponding to differential expression (log2 fold change) from AtFTOE 2.1 line vs WT Arabidopsis are visualized by Mapman (Fig. 3). Some data corresponding to down-regulated genes are presented in Table 3.

Determination of leaf number and rosette diameter in three AtFTOE and WT plants
Three AtFTOE (2.1,3.1 and 4.3) lines and WT Arabidopsis Columbia-0 ecotype were employed. Seeds were stratified, kept at 4°C for 3 days in the dark and then germinated and grown in hydroponic system [1] at 22°C under controlled conditions, initially in short days (8 h light, 16 h dark) and after 21 days the seedlings were transferred to long-day (16 h light, 8 h dark) photoperiod under 100-120 mmol m À 2 s À 1 . Plants grown under these conditions were used for determining rosette leaf     Table 2). The raw data files are available at the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) accession numbers SRR2094583 and SRR2094587 for AtFTOE replicates 1-3 and AtWT for control replicates 1-2 respectively. The paired-end reads were aligned to the reference Arabidopsis genome using Tophat (version 2.0.10) [7] and Bowtie2 (version 2.1.0) [4]. The reference Arabidopsis genome and gene model annotation files (TAIR10 oftp://igenome:G3nom3s4u@ussd-ftp.illumina.com/Arabi-dopsis_thaliana/NCBI/TAIR10/Arabidopsis_thaliana_NCBI_TAIR10.tar.gz 4) were downloaded from the Illumina iGenomes (http://support.illumina.com/sequencing/sequencing_software/igenome.html). Differential expression was determined by Cufflinks (version 2.1.1) as described by Trapnell et al. [8] and then was visualized by CummeRbound, an edgeR package [3]. To analyze the variation in expression between two replicates from WT and three replicates from AtFTOE it was calculated the absolute difference of the log2 fold change and adjusted to P-value r0.05.

Acknowledgments
This work was supported by Departmental funds from CINVESTAV-IPN, by CONACyT grants nos. 156162 to RR-M and 105985 to BX-C, and a SENASICA-SAGARPA grant to BX-C and RR-M. LD-B was Table 1 Primers to detect differentially expressed genes.