Qualitative and quantitative analysis data of the major constituents of Ilex paraguariensis leaves by UPLC-PDA and QTOF-MS

Ilex paraguariensis A. St. Hil. is a native plant of South America widely consumed as beverages for its ethno pharmacological properties, such as antioxidant, anti-inflammatory, hypocholesterolemic as well as its benefits on the cardiovascular system. Since these properties are related to its chemical composition, the identification and quantification of the major compounds of I. paraguariensis extracts still remains relevant. The data described in this article supports previous results on the anti-inflammatory effect of I. paraguariensis A. St. Hil (Mate), “The anti-inflammatory effect of I. paraguariensis A. St. Hil (Mate) in a murine model of pleurisy” [1]. The present data article reports on nine major compounds identified in I. paraguariensis extracts and its related fractions by using UPLC-PDA and UPLC-QTOF. Identification of the constituents was based on their retention times, UV absorption spectra and mass spectra data, as well as by comparison with authentic samples. The validated parameters show that the quantification by UPLC-PDA methodology developed is sensitive, precise and accurate.

fractions by using UPLC-PDA and UPLC-QTOF. Identification of the constituents was based on their retention times, UV absorption spectra and mass spectra data, as well as by comparison with authentic samples. The validated parameters show that the quantification by UPLC-PDA methodology developed is sensitive, precise and accurate.
& 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Pharmaceutical sciences More specific subject area Natural product chemistry; Pharmacognosy Type of data Figure and tables How data was acquired Ultra performance liquid chromatography with photo-diode array and mass spectrometry detection using an Acquity-UPLC™ (Waters, MA, USA) coupled to a high-resolution mass spectrometer (Xevo G2-S QTof model), equipped with an electrospray ionization source and controlled by MassLynx v.4.1 software.

Data format
Analyzed Experimental factors Hydroethanolic extract of Ilex paraguariensis leaves was prepared by turboextraction. The extract was dried (CE) and a portion was poured onto water and partitioned with n-BuOH, yielding the n-BuOH fraction (BF) and aqueous residual fraction (ARF). For the LC analysis, the samples were dissolved in the mobile phase and filtered through a 0.22 mm membrane before the injection in UPLC.

Experimental features
The extract and the fractions of Ilex paraguariensis leaves were analyzed qualitatively and quantitatively by UPLC-PDA and UPLC-MS.

Data source location
Department of Pharmaceutical Science, Centre of Health Sciences, Federal University of Santa Catarina, 88040-900, Florianopolis, Brazil.

Data accessibility
Data is with this article

Value of the data
Chromatographic and mass spectrometric data can be used for comparison with other studies performed on I. paraguariensis extracts.
The extract contents and the metabolites identification data will provide a valuable reference for studies comprising the chemical and pharmacological effects of I. paraguariensis.
Allows the characterization of new targets and new potential functions for this medicinal plant. Interaction network generated.

Data
The following dataset includes one figure and two tables that support the identification as well as validation assays to quantify the major compounds of Ilex paraguariensis samples extracts. Fig. 1 shows the UPLC-PDA chromatographic profile of I. paraguariensis crude extract. The chromatographic and spectroscopic (UPLC-PDA and UPLC-MS) detection parameters data for each identified compound are presented in Table 1. Further, Table 2 data shows the validated parameters of the methodology developed. The different contents data of compounds of I. paraguariensis crude extract and its related fractions are showed in Table 1 in Ref. [1].

Plant material
Plant material data described here has been carried out in accordance with Ref. [1]. After harvesting, I. paraguariensis A. St. Hil. leaves (RSPF 11074) were processed and stored until the extraction and the preparation of its related fractions.

Extract preparation
The hydroethanolic I. paraguariensis crude extract were prepared by turboextraction in an Ultra-Turrax s apparatus. After filtration, the solvent was removed under reduced pressure, and the obtained crude extract (CE) was partitioned between n-BuOH and water affording fractions BF and ARF, respectively. For details of the samples preparation see Ref. [1].

Chromatographic separation
All the chromatographic conditions are related to Ref. [1]. Chromatographic separation was achieved with an Acquity-UPLC™ (Waters s , MA, USA) system equipped with a quaternary pump, degasser, autosampler, photodiode array detector (PDA) and a Waters BEH C18 column, 1.7 μm, 50 Â 2.1 mm at 40°C as the stationary phase. The method used a gradient at constant flow rate (0.3 mL min À 1 ) combining solvent A (formic acid/water, pH 2.5) and solvent B (acetonitrile), programmed as follows: 0-5 min, linear change from A-B (97:3 v/v) to A-B (90:10 v/v); 5-6 min, isocratic A-B (90:10 v/v); 6-9 min, linear change to A-B (80:20 v/v) and 9-10 min, linear change to A-B (10:90 v/v). The peaks were characterized by comparing the retention time, UV spectra and by coinjection of the sample with the reference standards. Quantification was performed by external calibration curve, using their corresponding standards. Caffeine (Sigma-Aldrich s ) was quantified at 280 nm, while the phenolic compounds chlorogenic acid (Fluka s ) and rutin (Sigma-Aldrich s ) were quantified at 320 nm. All the analyses were performed in triplicate, and the peak area measured. Quantification was achieved using regression curves in the following ranges: 0.05-50 μg mL À 1 for the caffeine; 0.05-100 μg mL À 1 for chlorogenic acid and 0.25-50 μg mL À 1 for rutin. The regression equations were "y¼39320x þ2074.9" for caffeine, "y¼ 46696x À 1896" for chlorogenic acid, and "y ¼15618x þ178.92" for rutin. The extract was analyzed at a concentration of 500 μg mL À 1 and the injection volume was 5 μL.

UPLC-MS analysis
In addition to UPLC-PDA analysis, the identification was carried out by liquid chromatography (UPLC, Waters s Acquity mode) coupled to a high-resolution mass spectrometer (Xevo G2-S QTof model), equipped with an electrospray ionization probe. MassLynx v.4.1 software was used for data acquisition and processing. The mass spectrometer parameters were set as detailed in Ref. [1].

UPLC-PDA validation procedure
The UPLC-PDA quantification method was validated according to the ICH guidelines (2005) [6] for specificity, linearity, accuracy, precision (repeatability and intermediate precision), limit of quantification (LOQ) and limit of optical detection (LOD). The good linearity ranges were achieved by the analysis of linear correlation coefficient (caffeine, r 2 ¼1; chlorogenic acid, r 2 ¼0.9999; rutin, r 2 ¼0.9999) of the regression curves. The precision was determined by repeatability (intra-day assay) and intermediate precision (inter-day assay). The intra-day assay was performed in triplicate analysis of three different concentrations of the standard solutions, and expressed as relative standard deviation (RSD). The inter-day assay was determined by the analysis of a medium concentration in the curve, three times a day, on three different days. The limit of quantification (LOQ) and limit of detection (LOD) were defined by relative standard deviation (RSD o 5%) and by a signal:noise ratio of 3:1, respectively. Accuracy was determined by spiking samples with the standard solutions of caffeine, chlorogenic acid or rutin (1:1 v/v) and the average recovery values were calculated.