Data set in support of neurotoxicity of trimethyltin chloride by morphological and protein analysis

Trimethyltin chloride (TMT) is a neurotoxicant widely present in the aquatic environment. Chronic exposure of embryos to TMT for 4 days post-fertilization (dpf) elicited a concentration-related decrease in head & eye size and increase in axial malformation. In addition, Rohon-Beard sensory neurons and motor neurons showed decreased patterns of protein expression. These data coincide with previous research about the neurotoxicity of TMT on mRNA expression (Kim et al., 2016 [1]). These data demonstrates that TMT inhibits specific neurodevelopmental stages in zebrafish embryos and suggests a possible mechanism for the toxicity of TMT in vertebrate neurodevelopment. This paper contains data related to research concurrently published in Kim et al. (2016) [1].


a b s t r a c t
Trimethyltin chloride (TMT) is a neurotoxicant widely present in the aquatic environment. Chronic exposure of embryos to TMT for 4 days post-fertilization (dpf) elicited a concentration-related decrease in head & eye size and increase in axial malformation. In addition, Rohon-Beard sensory neurons and motor neurons showed decreased patterns of protein expression. These data coincide with previous research about the neurotoxicity of TMT on mRNA expression (Kim et al., 2016 [1]). These data demonstrates that TMT inhibits specific neurodevelopmental stages in zebrafish embryos and suggests a possible mechanism for the toxicity of TMT in vertebrate neurodevelopment. This paper contains data related to research concurrently published in Kim  These data show the dose-dependent effects of TMT on morphological defects in the head formation of zebrafish.
These data show the specific neurotoxicity of TMT on neurodevelopmental stage in zebrafish.

Data
Neurotoxicity of TMT acute exposure was not detected in early developmental stage. However, prolonged exposure of TMT induced abnormal morphology in zebrafish embryo (Fig. 1). In addition, chronic exposure of TMT elicited morphological defects in head including head & eye size in a dose dependent manner ( Fig. 2A, B). To evaluate the specific neurotoxicity, immunohistochemistry was performed on Rohon-Beard sensory neurons and motor neuron, which confirmed specific neurodevelopmental inhibition induced by TMT. At 10 μM, morphological differences in Rohon-Beard sensory neurons (zn-12) and motor neuron (znp-1) positive neurons were noted in ventrally projecting axons compared with the control group (Fig. 3A, B, D and E). Moreover, it was difficult to detect morphologic normality in axons or axon branches at 15 μM (Fig. 3C, F). This paper contains data related to research concurrently published in [1].

Embryo-larvae Toxicity assay
All the procedures using zebrafish embryos were approved from the Institutional Animal Care and Use Committee of Seoul National University. Embryos and larvae were monitored for morphological analysis at every 0.5 dpf for 4 days using an Olympus IX70 (Olympus, Japan) at 4 dpf.

Statistical analysis
A one-way analysis of variance (ANOVA) followed by the Tukey HSD test was performed in SPSS Statistics (version 16). Experimental data were checked to determine if there was a significant difference. The level for statistical significance was set at the 0.05 and 0.01.