Data of continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces

This data article contains two figures and one table supporting the research article entitled: “Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surface” [1]. The table shows coating conditions of three copolymers, poly(styrene-co-acrylic acid) grafted with oligovitronectin, poly(styrene-co-N-isopropylacrylamide) and poly(styrene-co-polyethylene glycol methacrylate) to prepare thermoresponsive surface. XPS spectra show the nitrogen peak of the polystyrene surface coated with poly(styrene-co-acrylic acid) grafted with oligovitronectin. The surface coating density analyzed from sorption of poly(styrene-co-acrylic acid) grafted with oligovitronectin by UV–vis spectroscopy is also presented.

12 December 2015 Accepted 28 December 2015 Available online 13 January 2016 detachment from thermoresponsive nanobrush surface" [1]. The table shows coating conditions of three copolymers, poly(styrene-coacrylic acid) grafted with oligovitronectin, poly(styrene-co-N-isopropylacrylamide) and poly(styrene-co-polyethylene glycol methacrylate) to prepare thermoresponsive surface. XPS spectra show the nitrogen peak of the polystyrene surface coated with poly(styreneco-acrylic acid) grafted with oligovitronectin. The surface coating density analyzed from sorption of poly(styrene-co-acrylic acid) grafted with oligovitronectin by UV-vis spectroscopy is also presented

Value of the data
The data show coating conditions of three copolymers, poly(styrene-co-acrylic acid) grafted with oligovitronectin, poly(styrene-co-N-isopropylacrylamide) and poly(styrene-co-polyethylene glycol methacrylate) on polystyrene tissue culture plates for the preparation of thermoresponsive surface.
The data show which concentration of the coating polymer is necessary to cover the surface. The surface coating density can be measured by spectroscopy on the surface coated with poly (styrene-co-acrylic acid) grafted with oligovitronectin The data show the evaluation of oligovitronectin measured by XPS spectra of the surface coated with poly(styrene-co-acrylic acid) grafted with oligovitronectin The existence of oligovitronectin on the surface coated with poly(styrene-co-acrylic acid) can be verified by XPS measurement.
Data Table 1

Experimental design, materials and methods
We designed three types of coating copolymers: (a) a stem cell binding site, (b) a thermoresponsive site, and (c) a hydrophilic site. Hydrophobic polystyrene (PSt) was selected as the anchoring site of these three copolymers on the surface of TCPS. For this purpose, we synthesized Table 1 Coating conditions of thermoresponsive surface used in this study.

Coating conditions
Coating concentration (mg/ml) Figure     were prepared by a reversible addition-fragmentation chain transfer (RAFT) polymerization. The synthesis method of these copolymers was described in Ref. [1] in detail.

Preparation process of thermoresponsive nanobrush surface
0-3 mg/mL of P[St-AA] in ethanol was added in TCPS dishes (4 cm 2 of surface area, 12 well dishes) for coating of P[St-AA] on the surface for 2 h at 25°C and subsequently removed from the dishes. TCPS dishes coated with P[St-AA] were activated via immersion in an aqueous solution containing 10 mg/ml N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and 10 mg/ml N-hydroxysuccinimide (NHS) for 1 h at 37°C after washing the dishes with phosphate buffered saline (PBS, pH 7.2) three times [1,4,5]. Subsequently, the dishes were washed with PBS and immersed in a PBS solution containing 1000 μg/mL of oli-goVN for 24 h at 4°C to prepare P[St-AA]-oligoVN dishes. The dishes were washed with PBS three times [1].

Characterization of dishes by XPS
The chemical composition of the dishes on the TCPS surface with P[St-AA]-oligoVN was analyzed using X-ray photoelectron spectroscopy (XPS, K-Alpha spectrometer, Thermal Scientific, Inc., Amarillo, TX, USA, equipped with a monochromatic Al-K X-ray source [1486.6 eV photons]). The energy of the emitted electrons was measured using a hemispherical energy analyzer at pass energies ranging from 50 to 150 eV. Data were collected at a photoelectron takeoff angle of 45°with respect to the sample surface. The binding energy (BE) scale was referenced by setting the peak maximum in the C1s spectrum to 284.6 eV. The obtained high-resolution C1s spectra were fitted using Shirley background subtraction and a series of Gaussian peaks [1,5].