Optimization of solid lipid nanoparticles prepared by a single emulsification-solvent evaporation method

This data article contains the data related to the research article “Characterization, biorecognitive activity and stability of WGA grafted lipid nanostructures for the controlled delivery of rifampicin” (Pooja et al. 2015) [1]. In the present study, SLN were prepared by a single emulsification-solvent evaporation method and the various steps of SLN preparation are shown in a flow chart. The preparation of SLN was optimized for various formulation variables including type and quantity of lipid, surfactant, amount of co-surfactant and volume of organic phase. Similarly, effect of variables related to homogezation, sonication and stirring processes, on the size and surface potential of SLN was determined and optimized.


a b s t r a c t
This data article contains the data related to the research article "Characterization, biorecognitive activity and stability of WGA grafted lipid nanostructures for the controlled delivery of rifampicin"   [1]. In the present study, SLN were prepared by a single emulsification-solvent evaporation method and the various steps of SLN preparation are shown in a flow chart. The preparation of SLN was optimized for various formulation variables including type and quantity of lipid, surfactant, amount of cosurfactant and volume of organic phase. Similarly, effect of variables related to homogezation, sonication and stirring processes, on the size and surface potential of SLN   Formulation and process parameters were changed for optimization of size and zeta potential of nanoparticles. Experimental features Various formulations were prepared by single emulsification-solvent evaporation method to get nanoparticles of desired size and zeta potential. Data source location NA Data accessibility The data are presented in this article

Value of data
The article describes the preparation, optimization and characterization of solid lipid nanoparticles. The data can be useful for other researchers investigating the effects of different lipids and surfactants on size and surface charge of nanoparticles.
The optimized formulation parameters could be used for the development of solid lipid nanoparticles of hydrophobic drugs.

Experimental design, material and methods
Solid lipid nanoparticles (SLN) i.e. lipid nanoparticles with solid matrix is the most fascinating carrier for oral drug delivery because of their excellent biocompatibility, high drug loading, long-term stability and feasibility for large scale production [1][2][3][4][5]. In this study, solid lipid nanoparticles (SLN) were prepared by a single emulsification-solvent evaporation method. Fig. 1 presents the various steps of preparation of SLN. Various formulation parameters (Table 1) and process variables ( Table 2) were optimized on the basis of their effect on particle size, polydispersity index and zeta potential.

. Type and quantity of lipids
Three different lipids viz. glyceryl monostearte (GMS), tristearin and tripalmitin were used as lipid matrix. The particle diameter (PD), polydispersity index (PDI) and zeta potential (ZP) were measured using a Zetasizer NanoZS (Malvern, UK). The lipid showing minimum PD and PDI was selected and used in three different quantities (80, 100 and 120 mg).

Type and concentration of surfactants
The type and concentration of surfactant affect the particle size as well as stability of nanoparticles. At low concentration, surfactant will not be sufficient to cover the surface of nanoparticles resulting into increased particle size due to particle aggregation. High concentration of surfactant may lead to bridging between nanoparticles and may also cause toxicity. Therefore, three different surfactants (Tween s 80, Poloxomer 188 and polyvinyl alcohol) were evaluated at three different concentrations (1%, 1.5% and 2% w/v).

Volume of organic phase
The organic solvent is used to dissolve the lipids and chloroform was used in this study in varying volumes (1-5 mL). The formulation showing good particle size with minimum volume of solvent was selected.

Quantity of co-surfactant
Lecithin soy was used as co-surfactant which act as internal emulsifier and favors to particle size reduction and stability. Lecithin soy was used at different concentration (20, 30 and 40) to get a formulation having small particle size, less PDI with good zeta potential and stability. The organic phase was poured in aqueous surfactant phase and homogenized at different speed (5000, 8000 and 11000 rpm) for different time (3, 4, 5 and 6 min) to get course emulsion. Then this course emulsion was sonicated for different time period to get a nanoemulsion. Finally formulation was stirred to evaporate the organic solvent and to get the nanoparticles. The formulation was stirred at different speed (800, 1000, and 1200 rpm) and for different time period (1, 2 and 3 h) for optimization.