Notes
Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese,☆☆

https://doi.org/10.1016/j.diagmicrobio.2011.06.016Get rights and content

Abstract

Rapid diagnosis of individuals involved in brucellosis outbreaks can sometimes be difficult with conventional microbiological techniques. We analyzed, for the first time, the diagnostic yield of a real-time polymerase chain reaction (PCR) assay in a family outbreak of brucellosis due to consumption of unpasteurized goat cheese. PCR correctly identified all symptomatic cases.

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Acknowledgments

The authors thank Ian Johnstone for his help with the English language version of the text.

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Funding: This work received financial support from the Instituto de Salud Carlos III (ISCIII), F.I.S (grant PI06/0495) and the Consejería de Innovación Ciencia y Empresa (grants CTS-276 and P-08 CTS 3969), both of the Junta de Andalucía (Spain).

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Potential conflicts of interest: All authors read and approved the final manuscript.

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