Original articleHelicobacter pylori BabA Expression, Gastric Mucosal Injury, and Clinical Outcome
Section snippets
Helicobacter pylori
We examined H pylori isolates cultured from patients in East Asia and North and South America with clinical presentations including simple H pylori gastritis, duodenal ulcer (DU), and noncardiac gastric adenocarcinoma. Ulcers were defined endoscopically, and patients with either ulcer scars or DU and gastric ulcers were excluded. Gastritis was defined as histologic gastritis with no peptic ulcers or gastric cancer. No patients received treatment for their H pylori infection, and those patients
Lewis b Antigen Binding Assay
We first evaluated the accuracy of assessing BabA status by immunoblotting and PCR analysis as compared with assessing Leb binding activity. Eighty H pylori strains from our Colombian and Japanese H pylori stocks (40 strains from each country) were examined. Leb binding activity was detectable in 68 (85%) of these strains (83% of the Colombian strains and 88% of the Japanese strains).
Comparison Between BabA Immunoblotting and Lewis b Antigen Binding Assay
Based on the results of immunoblotting analyses, the H pylori strains were divided into 2 major groups: BabA
Discussion
We show that PCR-based methods designed to detect a 10-bp deletion in the signal region of the babA gene do not reliably reflect BabA expression as determined by immunoblotting or Leb binding activity. These results call into question the conclusions of previous studies using those techniques to relate BabA functional status and histology or clinical outcome.
BabA-negative status is associated with mild gastric injury and lower H pylori density. BabA-negative strains also are associated
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Cited by (0)
This report is based on work supported in part by grants from the National Institutes of Health (R01 DK62813 to Y.Y.), the Office of Research and Development, Medical Research Service Department of Veterans Affairs (to D.Y.G.), the Deutsche Forschungsgemeinschaft (project OD 21/1-1) (to S.O.), and a Public Health Service grant (DK56338) that funds the Texas Gulf Coast Digestive Diseases Center. O.O.O. was supported by a UNESCO BAC short-term fellowship and a Macarthur Foundation/University of Ibadan Staff Training Grant.
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Current addresses: O.O.O.: Department of Microbiology, University College Cork, Cork, Republic of Ireland; J.Y.W.: Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.