Cell Reports
Volume 26, Issue 6, 5 February 2019, Pages 1668-1678.e4
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Microcolony Size Distribution Assay Enables High-Throughput Cell Survival Quantitation

https://doi.org/10.1016/j.celrep.2019.01.053Get rights and content
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Highlights

  • The MicroColonyChip (μCC) is a rapid cell survival quantitation platform

  • Colony micropatterning enables 250× miniaturization of the colony formation assay

  • Analysis of microcolony sizes enables rapid automated analysis of cell survival

  • μCC is more sensitive and robust than commonly used cytotoxicity assays

Summary

Cell survival is a critical and ubiquitous endpoint in biology. The broadly accepted colony formation assay (CFA) directly measures a cell’s ability to divide; however, it takes weeks to perform and is incompatible with high-throughput screening (HTS) technologies. Here, we describe the MicroColonyChip, which exploits microwell array technology to create an array of colonies. Unlike the CFA, where visible colonies are counted by eye, using fluorescence microscopy, microcolonies can be analyzed in days rather than weeks. Using automated analysis of microcolony size distributions, the MicroColonyChip achieves comparable sensitivity to the CFA (and greater sensitivity than the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide [XTT] assay). Compared to CellTiter-Glo, the MicroColonyChip is as sensitive and also robust to artifacts caused by differences in initial cell seeding density. We demonstrate efficacy via studies of radiosensitivity and chemosensitivity and show that the approach is amenable to multiplexing. We conclude that the MicroColonyChip is a rapid and automated alternative for cell survival quantitation.

Keywords

colony formation assay
cell proliferation
miniaturization
fluorescence microscopy
high-throughput cytotoxicity assay
anti-cancer efficacy
drug safety
cytotoxicity
multiplex assay

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