Cell
Volume 141, Issue 2, 16 April 2010, Pages 344-354
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Theory
An Evolutionarily Conserved Mechanism for Controlling the Efficiency of Protein Translation

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Summary

Recent years have seen intensive progress in measuring protein translation. However, the contributions of coding sequences to the efficiency of the process remain unclear. Here, we identify a universally conserved profile of translation efficiency along mRNAs computed based on adaptation between coding sequences and the tRNA pool. In this profile, the first ∼30–50 codons are, on average, translated with a low efficiency. Additionally, in eukaryotes, the last ∼50 codons show the highest efficiency over the full coding sequence. The profile accurately predicts position-dependent ribosomal density along yeast genes. These data suggest that translation speed and, as a consequence, ribosomal density are encoded by coding sequences and the tRNA pool. We suggest that the slow “ramp” at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression.

Highlights

► The efficiency of translation is universally lower across the first ∼50 codons ► Evolutionary forces act to maintain this profile of translation elongation speed ► The profile is predictive of ribosome density for translation of yeast genes ► The ramp in efficiency may contribute to fitness and managing the cost of translation

PROTEINS

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5

These authors contributed equally to this work