Elsevier

Bioelectrochemistry

Volume 144, April 2022, 107981
Bioelectrochemistry

Reduction of plasmid vector backbone length enhances reporter gene expression

https://doi.org/10.1016/j.bioelechem.2021.107981Get rights and content
Under a Creative Commons license
open access

Highlights

  • Reduced vector size increases gene expression in vitro.

  • Reduced vector size enhances gene delivery to several layers of the skin.

  • Reduced vector size enhances gene delivery to the myocardium.

Abstract

Gene therapy has a wide range of applications for various types of pathologies. Viral methods of gene delivery provide high levels of gene expression but have various safety concerns. Non-viral methods are largely known to provide lower levels of expression. We aim to address this issue by using plasmid DNA with smaller backbones to increase gene expression levels when delivered using non-viral methods. In this study we compare gene expression levels between two vectors with firefly luciferase encoding gene insert using liposome complexes and gene electrotransfer as delivery methods. A 2-fold reduction in plasmid vector backbone size, disproportionately enhanced gene expression levels more than 10-fold in rat tenocytes in vitro, and rat myocardium in vivo, while improvements in delivery to the skin were more moderate.

Keywords

Plasmid DNA
Gene expression
Gene therapy
Gene electrotransfer

Cited by (0)