Biochemical and Biophysical Research Communications
The expression of inhibin beta B is high in human adipocytes, reduced by weight loss, and correlates to factors implicated in metabolic disease
Section snippets
Materials and methods
Subjects and samples. Adipose tissue biopsies from the abdominal subcutaneous depot and the major omentum were obtained from nine obese men [3], [11] and four obese women [3] undergoing laparoscopic bariatric surgery. Adipocytes were prepared from subcutaneous and omental adipose tissue as previously described [12]. Abdominal subcutaneous adipose tissue biopsies were obtained from 32 obese subjects before (week 0), during (week 8 or weeks 8/16), and after (week 18) diet-induced weight loss,
Identification of genes predominantly expressed in subcutaneous adipocytes
Using DNA microarray datasets from 33 human tissues and cells, 25 genes were identified as predominantly expressed in subcutaneous adipocytes (Table 2). A large fraction comprised of genes previously known to be highly expressed in adipose tissue (e.g., adipose specific 2, hormone-sensitive lipase, aquaporin 7, PPARγ, etc.), whereas others were more unexpected (e.g., INHBB).
INHBB mRNA expression in adipose tissue
The expression of INHBB has not previously been reported in human adipose tissue and the role for INHBB in adipose tissue
Discussion
In this study, we have identified genes that are predominantly expressed in human subcutaneous adipocytes. We were surprised to find that INHBB mRNA, coding for the activin βB subunit, was highly expressed in adipocytes and that INHBB mRNA levels in adipose tissue were down regulated during diet-induced weight loss. Furthermore, INHBB expression in adipose tissue during diet-induced weight loss was highly correlated to risk factors included in the definition of the metabolic syndrome [25], such
Acknowledgments
This work was supported by grants from The Swedish Research Council 11285, 529-2002-6671, 521-2002-6356, and 521-2005-6736, the IngaBritt and Arne Lundberg research foundation, the Fredrik and Ingrid Thuring foundation, the Magn. Bergvall foundation, the Tore Nilson foundation for Medical Research, the National Board of Health and Welfare, the Wilhelm and Martina Lundgren Science foundation, and the Adlerbertska research foundation. VLCD was provided by Cambridgekuren Sverige AB. We thank
References (35)
Regulation of cellular and system function by activin
Biochem. Pharmacol.
(1998)- et al.
Comparison of two methods for determining human adipose cell size
J. Lipid Res.
(1972) - et al.
Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction
Anal. Biochem.
(1987) - et al.
A web-accessible complete transcriptome of normal human and DMD muscle
Neuromuscul. Disord.
(2002) - et al.
Identification of genes predominantly expressed in human macrophages
Atherosclerosis
(2004) - et al.
Monoclonal antibody based ELISAs for measurement of activins in biological fluids
J. Immunol. Methods
(1993) - et al.
The metabolic syndrome
Lancet
(2005) - et al.
Activin A inhibits differentiation of 3T3-L1 preadipocyte
Mol. Cell. Endocrinol.
(2005) - et al.
Adipose tissue as an endocrine organ
J. Clin. Endocrinol. Metab.
(2004) - et al.
Visfatin: a protein secreted by visceral fat that mimics the effects of insulin
Science
(2005)
A microarray search for genes predominantly expressed in human omental adipocytes: adipose tissue as a major production site of serum amyloid A
J. Clin. Endocrinol. Metab.
Regional differences of insulin action in adipose tissue: insights from in vivo and in vitro studies
Acta Physiol. Scand.
Regional difference in insulin inhibition of non-esterified fatty acid release from human adipocytes: relation to insulin receptor phosphorylation and intracellular signalling through the insulin receptor substrate-1 pathway
Diabetologia
Regional adiposity and insulin resistance
J. Clin. Endocrinol. Metab.
Visceral fat and insulin resistance—causative or correlative?
Br. J. Nutr.
Inhibins, activins, and follistatins: the saga continues
Proc. Soc. Exp. Biol. Med.
Roles of activin and its signal transduction mechanisms in reproductive tissues
Reproduction
Cited by (45)
Regulatory expression of components in the BMP pathway in white adipose tissues of cattle
2015, Livestock ScienceCitation Excerpt :In humans, the expression levels of components in the BMP pathway in adipose tissue appear to be related to adiposity. The expression level of activin βB in adipose tissue was previously shown to be higher in obese humans than in lean humans, and was decreased by reductions in body weight (Sjöholm et al., 2006). A negative relationship has been reported between BMP4 expression levels in adipose tissue and body mass index (Qian et al., 2013).
Transcriptome analysis of human adipocytes implicates the NOD-like receptor pathway in obesity-induced adipose inflammation
2014, Molecular and Cellular EndocrinologyRoles of activin family in pancreatic development and homeostasis
2012, Molecular and Cellular EndocrinologyPituitary gonadotropins FSH and LH are oppositely regulated by the activin/follistatin system in a basal teleost, the eel
2012, General and Comparative EndocrinologyActivin in Glucose Metabolism
2011, Vitamins and Hormones
- 1
These authors contributed equally.