Biochemical and Biophysical Research Communications
Upregulation of ICOS on CD43+ CD4+ murine small intestinal intraepithelial lymphocytes during acute reovirus infection
Section snippets
Materials and methods
Mice and reagents. Adult female C57BL/6 mice, 6–8 weeks of age, were purchased from Harlan Spargue–Dawley; Indianapolis, IN. Mice were housed under specific pathogen-free conditions. Animals were used according to protocols approved by the Institutional Animal Care and Use Committee of the University of Texas Health Science Center at Houston. Cell culture medium consisted of RPMI-1640 supplemented with FCS (10% v/v) (Invitrogen; Carlsbad, CA), 100 U/ml penicillin–streptomycin, 2 mM l-glutamine,
During early phase of reovirus infection, actively proliferating IELs include both S7− and S7+ cells
Recent studies from our laboratory demonstrated the CD43 S7 isoform is differentially expressed on small intestinal IELs [13]. That property is also shown in the present study in Fig. 1A, which indicates that roughly equal proportions of IELs were S7− and S7+, and that each population included both TCRαβ and TCRγδ cells.
To understand the relationship between CD43 S7 expression during enteric virus infection, mice were infected with 107.5 pfu of reovirus 3 by oral gavage. Forty-eight hours prior
Discussion
Owing to their phenotypic and functional complexity [13], [31], [32], [33], [34], [35], [36], [37], [38], [39], understanding the contribution of intestinal IELs to the natural immune response during local antigen challenge has been difficult to fully assess. In a recent series of studies aimed at better understanding functional populations of IELs, we observed that murine IELs can be classified based on whether they express a CD43 glycoform recognized by mAb S7. Accordingly, S7+ IELs were
Acknowledgments
This work was supported by NIH Grant DK35566 and by Public Health Service Grant DK56338 to the Texas Gulf Coast Digestive Diseases Center.
References (48)
- et al.
Regulatory role of CD43 leukosialin on integrin-mediated T-cell adhesion to endothelial and extracellular matrix ligands and its polar redistribution to a cellular uropod
Blood
(1995) - et al.
Comparative analysis of T-cell costimulation and CD43 activation reveals novel signaling pathways and target genes
Blood
(2004) - et al.
Stimulation via the CD43 coreceptor augments T cell proliferation during the early phase of antigen-induced activation
Dev. Comp. Immunol.
(1996) - et al.
Mucosal and systemic immunity to intestinal reovirus infection in aged mice
Exp. Gerontol.
(2004) - et al.
An improved method for isolating intraepithelial lymphocytes (IELs) from the murine small intestine with consistently high purity
J. Immunol. Methods
(2006) - et al.
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
Methods
(2001) - et al.
The CD28-related molecule ICOS is required for effective T cell-dependent immune responses
Immunity
(2000) - et al.
Targeting of inducible costimulator (ICOS) expressed on alloreactive T cells down-regulates graft-versus-host disease (GVHD) and facilitates engraftment of allogeneic bone marrow (BM)
Blood
(2005) - et al.
Biological insights into TCRγδ+ and TCRαβ+ intraepithelial lymphocytes provided by serial analysis of gene expression (SAGE)
Immunity
(2001) - et al.
Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine
Vet. Immunol. Immunopathol.
(2000)
Ameliorating effect of anti-inducible costimulator monoclonal antibody in a murine model of chronic colitis
Gastroenterology
Hyperexpression of inducible costimulator and its contribution on lamina propria T cells in inflammatory bowel disease
Gastroenterology
CD43, a molecule with multiple functions
Immunol. Res.
Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation
J. Immunol.
Expression of CD43 on murine and human pluripotent hematopoietic stem cells
J. Immunol.
Intracellular analysis of interleukin-2 induction provides direct evidence at the single cell level of differential coactivation requirements for CD45RA+ and CD45RO+ T cell subsets
J. Interferon Cytokine Res.
Modeling TCR signaling complex formation in positive selection
J. Immunol.
Dynamic regulation of T cell immunity by CD43
J. Immunol.
CD43 is a murine T cell costimulatory receptor that functions independently of CD28
J. Exp. Med.
Costimulation of host T lymphocytes by a trypanosomal trans-sialidase: involvement of CD43 signaling
J. Immunol.
CD43 potentiates CD3-induced proliferation of murine intestinal intraepithelial lymphocytes
Immunol. Cell Biol.
Most murine CD8+ intestinal intraepithelial lymphocytes are partially but not fully activated T cells
J. Immunol.
Mucosal T cell response to reovirus
Immunol. Res.
Reovirus receptors and pathogenesis
J. Virol.
Cited by (10)
Involvement of Ly6C, 4-1BB, and KLRG1 in the activation of lamina propria lymphocytes in the small intestine of sanroque mice
2017, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Due to the inability to recover IELs from sanroque mice and the presence of sufficient numbers of LPLs, experiments were conducted to assess the expression of activation markers on LPLs. Our previous studies demonstrated that CD43 [13,14], Ly6C (CD59) [15], CD69 [15], OX40 (CD134) [16], 4-1BB (CD137) [17], By55 (CD160) [18], ICOS (CD278) [12,19], and IL-18 and the IL-18R [20] are expressed in the small intestinal mucosa. Two of the most interesting of those, and the least studied to the present, are Ly6C and 4-1BB.
The drug monosodium luminol (GVT) preserves crypt-villus epithelial organization and allows survival of intestinal T cells in mice infected with the ts1 retrovirus
2009, Immunology LettersCitation Excerpt :These findings point to a local epithelial-lymphocyte hormone loop, involving TSH produced by crypt epithelial cells, response to this TSH by intestinal T cells, and feedback to the epithelium [37–39]. Intestinal epithelial TSH gradient is disturbed during rotavirus [38] and reovirus infection [39]. In both conditions, blocks of enterocytes on the villi reinitiate TSH expression, and groups of LP lymphoid cells aggregate in the LP below them.
Soluble gp130 promotes intestinal epithelial hyperplasia during reovirus infection
2010, International Journal of Experimental PathologyICOS promotes IL-17 synthesis in colonic intraepithelial lymphocytes in IL-10<sup>-/-</sup> mice
2010, Journal of Leukocyte Biology