Biochimica et Biophysica Acta (BBA) - Biomembranes
Volume 1828, Issue 10, October 2013, Pages 2339-2346
ReviewFTIR spectroscopic imaging of protein aggregation in living cells☆
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Graphical abstract
Highlights
► We review FTIR spectroscopy for evaluating the secondary structure of proteins. ► We describe its use for investigating protein misfolding and aggregation in vitro. ► New technology has enabled determination of aggregate structure in cells and tissue. ► Time-lapsed FTIR imaging of protein aggregation within living cells is demonstrated. ► Results can reveal the structural intermediates and mechanisms of cell toxicity.
Keywords
Fourier transform infrared (FTIR) spectroscopy
Microspectroscopy
Protein secondary structure
Protein aggregation
Amyloid
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This article is part of a Special Issue entitled: FTIR in membrane proteins and peptide studies.
Copyright © 2013 Elsevier B.V.