ORIGINAL ARTICLECombinatorial mutation on the β-glycosidase specific to 7-β-xylosyltaxanes and increasing the mutated enzyme production by engineering the recombinant yeast
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Graphical abstract
The combinatorial mutation strategy was employed to increase the β-glycosidase activity, and the mutant E12 with the highest enzyme activity was obtained. The E12 production in the yeast was further improved by co-expressing the molecular chaperone PDI (protein disulfide isomerase) and the Vitreoscilla hemoglobin under the promoter PAOX1. This engineered yeast can be used for the biocatalysis of 7-β-xylosyl-10-deacetyltaxol to 10-deacetyltaxol for Taxol semi-synthesis.
KEY WORDS
β-Glycosidases
Combinatorial mutation
Improved catalytic property
Molecular docking
Engineered yeast
Taxol
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