General Obstetrics and Gynecology: ObstetricsMechanisms of abruption-induced premature rupture of the fetal membranes: Thrombin enhanced decidual matrix metalloproteinase-3 (stromelysin-1) expression
Section snippets
Isolation of DCs
After receiving written informed consent, fetal membrane specimens were obtained after repeat cesarean deliveries from patients with uncomplicated pregnancies delivering at term either at Bellevue Hospital or Yale-New Haven Hospital under investigational review board and human investigation committee approval, respectively. The decidua was scraped from the maternal surface of the chorion and then minced and digested in Ham's F-10 plus 10% stripped calf serum (SCS) containing 25 mg/mL of
Statistical analysis
Comparisons of control versus treatment groups were performed using the analysis of variance test with P < .05 representing statistical significance.
Effects of steroids and thrombin on MMP-3 protein expression in term DCs
Figure 1 indicates that in 7 separate DC preparations, secreted levels of MMP-3 were reduced by more than 3- fold in cultures incubated in E2 plus MPA, compared with cultures maintained in E2 (n = 7; P < .05). Thrombin (2.5 U/mL) elevated MMP-3 output by 2.6-fold (P < .05) in both E2 and E2 plus MPA-treated cultures. The addition of MPA inhibited the effects of thrombin on MMP-3 output by reducing MMP-3 levels by about 70% (P < .05), compared with parallel cultures in which thrombin was added
Comment
The current study revealed that MMP-3 mRNA and protein expression in cultured term DCs is progestin inhibited and that thrombin reverses this inhibition. Previously, we found that constitutively expressed PAR1 was involved in mediating thrombin-enhanced plasminogen activator expression in decidualized stromal cells from cycling endometrium.22 To determine whether PAR1 also plays a role in thrombin-enhanced MMP-3 expression in term DCs, the current study evaluated the effects of TRAP-14, which
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