Original articleEvaluation of In Vitro Efficacy of Combined Riboflavin and Ultraviolet A for Acanthamoeba Isolates
Section snippets
Amoebic Isolates
The following strains of Acanthamoeba species were used as amebic isolates: Acanthamoeba species 65 was used as an environmental amoeba isolated from superficial water, and Acanthamoeba species 7376 was used as a clinical strain isolated via corneal scraping from a patient with keratitis. The 2 isolates were identified through the microscopic examination of their trophozoites and cysts (Figure 1). The keys reported by Poussard and Pons and by Page were used.19, 20 To confirm the identification
Results
In all groups and for both isolates studied (environmental and pathogenic strains), cysts and trophozoites were detected at distances from the center of the point of application of more than 5 mm after incubation at 30 C in the first evaluation (24 hours), so no re-evaluation or reculture was necessary because of the early growth. This indicated the viability of the amoebae tested. Trophozoites were found at a similar distance from the center of the point of application for all treatment groups
Discussion
In 2008, Iseli and associates used cross-linking to treat 5 patients with infectious keratitis associated with corneal melting,24 but reported only a bacterial or fungal cause. Corneal melting was halted after cross-linking treatment in all the cases. Recently, Morén and associates and Makdoumi and associates also reported that cross-linking therapy was successful in combating severe keratitis of unknown origin.25, 26
The efficacy of ultraviolet light as a disinfectant in contact lens, water, or
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