Elsevier

Analytical Biochemistry

Volume 411, Issue 2, 15 April 2011, Pages 303-305
Analytical Biochemistry

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Explicit analytic approximations for time-dependent solutions of the generalized integrated Michaelis–Menten equation

https://doi.org/10.1016/j.ab.2011.01.016Get rights and content

Abstract

Various explicit reformulations of time-dependent solutions for the classical two-step irreversible Michaelis–Menten enzyme reaction model have been described recently. In the current study, I present further improvements in terms of a generalized integrated form of the Michaelis–Menten equation for computation of substrate or product concentrations as functions of time for more real-world, enzyme-catalyzed reactions affected by the product. The explicit equations presented here can be considered as a simpler and useful alternative to the exact solution for the generalized integrated Michaelis–Menten equation when fitted to time course data using standard curve-fitting software.

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Acknowledgment

This study was supported by the Slovenian Research Agency (Grant P1-170).

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