Experimental transplantationThe technique for heterotopic cardiac transplantation in mice: experience of 3000 operations by one surgeon☆
Section snippets
Materials and methods
Mice, CBA (H2k), C3H (H2k), C57BL/10 (H2b), or CBK (H2k+Kb), Kb transgenic mice in the context of CBA (8), were purchased from Sankyo (Tokyo, Japan) and were housed in conventional facilities of Teikyo University or the University of Oxford and used between 8 and 12 weeks old in accordance with the Animals Scientific Procedures.
Improving operation time
More than 3000 heterotopic cardiac transplantations have been performed by one surgeon since 1994. The first successful transplantation was done after 20 trials and a 90% successful rate was achieved after 200 operations (Table 1). Operation time was decreased according to the number of operations and finally lasted 35 minutes per 1 operation (Figure 4). Success rate increased to 98% (Figure 5). The operation technique that was described on this paper was established after 1000
Discussion
To investigate transplantation study, small animal model is essential. Especially, mouse model is useful because of the many kinds of transgenic and gene knockout mice. However, heterotopic cardiac transplantation of mouse is much more difficult than that of a rat. Therefore, one of the simplest techniques was presented in this paper. The major point is the number of continuous stitches. First, 10 stitches on one side of aorta and IVC were taken. Too many sutures took a lot of time and annoyed
References (9)
- et al.
Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes
Transpl Immunol
(1998) - et al.
Evidence that non-deletional mechanisms are responsible for inducing and maintaining unresponsiveness after intrathymic injection of non-professional antigen presenting cells
J Heart Lung Transplant
(2000) - et al.
Primarily vascularized allografts of hearts in micethe role of H-2D, H-2K and non H-2 antigens in rejection
Transplantation
(1973) - et al.
Nondepleting anti-CD4 monoclonal antibody enhances the ability of oral alloantigen delivery to induce indefinite survival of cardiac allograftsoral tolerance to alloantigen
Transplantation
(2000)
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