A new species of Ophiostoma with a Leptographium anamorph from Larch in Japan

doi:10.1016/S0953-7562(09)81217-3

Mycological Research

Volume 99, Issue 11, November 1995, Pages 1334-1338

https://doi.org/10.1016/S0953-7562(09)81217-3 Get rights and content

Recent surveys of felled Larix logs infested with Ips cembrae in the Mount Fuji area of Japan have yielded numerous ophiostomatoid fungi. One of these Ophiostoma species superficially resembles Ophiostoma penicillatum in having allantoid ascospores with sheaths. However, the conidia of the Leptographium anamorph are small obovoid, and distinct from those of O. penicillatum, which are characteristically large, and cylindrical to allantoid. On the basis of the morphologically distinct anamorphs, we conclude that this collection from Larix represents a new Ophiostoma holomorph. It is consequently described as Ophiostoma laricis, anamorph, Leptographium laricis.

References (28)

MinterD.W. et al.
Holoblastic phialides
Transactions of the British Mycological Society
(1982)
MinterD.W. et al.
Thallic phialides
Transactions of the British Mycological Society
(1983)
SpataforaJ.W. et al.
The polyphyletic origins of ophiostomatoid fungi
Mycological Research
(1994)
Van WykP.S. et al.
Differences in synchronisation of stages of conidial development in Leptographium species
Transactions of the British Mycological Society
(1988)
WingfieldM.J.
Reclassification of Verticicladiella based on conidial development
Transactions of the British Mycological Society
(1985)
WingfieldM.J. et al.
Analysis of conidium ontogeny in anamorphs of Ophiostoma: Pesotum and Phialographium are synonyms of Graphium
Mycological Research
(1991)
BrasierC.M.
Ophiostoma novo-ulmi sp. nov., causative agent of current Dutch elm disease pandemics
Mycopathologia
(1991)
DavidsonR.W. et al.
A restudy of Ceratocystis penicillata and report of two American species of this genus from Europe
Mycologia
(1967)
De HoogG.S. et al.
Ceratocystis versus Ophiostoma: a reappraisal
Mycologia
(1984)
DowdingP.
The evolution of insect-fungus relationships in the primary invasion of forest timber

GibbsJ.N.

Intercontinental epidemiology of Dutch elm disease

Annual Review of Phytopathology

(1978)

HarringtonT.C.

Cycloheximide sensitivity as a taxonomic character in Ceratocystis

Mycologia

(1981)

HarringtonT.C. et al.

Leptographium root diseases on conifers

(1988)

HausnerG. et al.

On the subdivision of Ceratocystis s.l., based on partial ribosomal DNA sequences

Canadian Journal of Botany

(1992)

Cited by (21)

Taxonomy and pathogenicity of ophiostomatoid fungi associated with bark beetles infesting conifers in Japan, with special reference to those related to subalpine conifers
2017, Mycoscience
Citation Excerpt :
Ceratocystis fujiensis was transferred to the genus Endoconidiophora. Grosmannia laricis was first described by Van der Westhuizen et al. (1995) as O. laricis Van der Westh., Yamaoka & M.J. Wingf. Zipfel et al. (2006) conducted a molecular phylogenetic study, and separated Grosmannia from Ophiostoma.
Ophiostomatoid fungi are ambiguous. They belong to the Ascomycota, and were formerly treated as one family and grouped in one genus, but molecular phylogenic studies have shown that they are divided into two orders and their generic classification must be altered. Our research group conducted taxonomic studies on ophiostomatoid fungi, their association with bark beetles, and their pathogenicity against host trees. This review summarizes recent changes in the taxonomic treatment of these fungi and their relationships with bark beetles associated with subalpine conifers in Japan. Furthermore, methods for evaluating their virulence against conifer trees are discussed.
Phylogenetic relationship of Japanese isolates belonging to the Grosmannia piceiperda complex (Ophiostomatales)
2016, Mycoscience
Citation Excerpt :
Some studies proposed that G. piceiperda and G. europhioides are conspecific (Upadhyay 1981; Jacobs et al. 2000), while Solheim (1986), Harrington and Cobb (1988), and Yamaoka et al. (1997) treated them as distinct. Grosmannia laricis was described by Van der Westhuizen et al. (1995) from Ips subelongatus (as I. cembrae) infesting Larix kaempferi in Japan. This species is characterized by curved ascospores instead of cucullate ascospores.
This study was conducted to explore the morphological and phylogenetic relationships of the Japanese isolates tentatively identified as the Grosmannia piceiperda complex (Ophiostomatales, Ascomycota; asexual morph = Leptographium s.l.). The phylogenetic analyses of the internal transcribed spacer 2 and nuclear large subunit ribosomal RNA gene regions showed that the Japanese isolates belong to the G. piceiperda complex. The analyses of the partial β-tubulin and translation elongation factor-1 alpha (EF-1α) genes separated the species complex into nine and 13 lineages, respectively. Based on the β-tubulin sequences, morphologically distinct groups were included in the same clades. However, based on the EF-1α sequences, these groups could be separated into different lineages. Detailed morphological observations supported this result and confirmed that the Japanese lineages are distinct. Among the 13 recognized lineages, the Japanese isolates were separated into 11 lineages, including G. aenigmatica, G. laricis, G. piceiperda D, as well as eight lineages representing undescribed taxa.
Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs
2009, Mycoscience
Ceratocystiopsis, Ceratocystis, Grosmannia, and Ophiostoma species were isolated from Ips subelongatus and beetle-infested Japanese larch logs collected at several areas in central and northern Honshu Island, Japan, to determine constant associates of I. subelongatus. Ceratocystiopsis minuta, two species of Ceratocystis, three species of Grosmannia, and four species of Ophiostoma were isolated. Of the fungi isolated in the present study, G. laricis, O. brunneociliatum, and O. piceae were constant associates of the beetles. Ceratocystis fujiensis, Ceratocystiopsis minuta, and Ophiostoma sp. F were occasionally isolated with high frequencies of occurrence but were not consistent associates. Ceratocystis fujiensis was most often isolated as the leading fungal invasion in the sapwood of Japanese larch logs invaded by I. subelongatus, confirming that the fungus acts as a primary invader of sapwood in beetle-attacked logs.
New Leptographium species from Indonesia and Eastern North America
2000, Mycoscience
Leptographium species have predominantly been described from North America, Canada and Europe. These fungi generally occur on conifers and many cause blue-stain of lumber. Most Leptographium species are also associated with insects and in particular, bark beetles (Coleoptera: Scolytidae). Recently, an unknown species of Leptographium was isolated from pine infested with an Ips sp. in Indonesia. In addition, two unknown species have been collected from red spruce (Picea rubra) and balsam fir b4bies balsamea) roots from high elevation sites in Eastern North America. The latter isolates are unusual in that they are associated with the feeding wounds made by the conifer swift moth Korscheltellus gracilus (Lepidoptera: Hepialidae), which is a habitat unique for species of Leptographium. Comparison with known Leptographium species has revealed that the isolates from Indonesia and those from Eastern North America represent three previously undescribed taxa. They are, therefore, described in this study as L pineti sp. nov, L abieticolens sp. nov. and L. peucophilum sp. nov.
A new Leptographium species from Russia
2000, Mycological Research
Species of Leptographium are well-known inhabitants of conifers in the Northern Hemisphere, in which they cause a blue-stain. They are also known to be associated with insects, especially bark beetles (Coleoptera: Scolytidae). Surveys of dying stands of Siberian fir (Abies sibirica) have resulted in the consistent isolation of an unknown Leptographium from the galleries of the fir sawyer beetle, Monochamus urussovi (Coleoptera: Cerambycidae). This fungus is responsible for the blue-stain in living trees. Comparison with known species of Leptographium led to the conclusion that it had not been previously described, and the name Leptographium sibiricum sp. nov. is introduced here.
Comparison of Ophiostoma huntii and O. europhioides and description of O. aenigmaticum sp. nov.
1998, Mycological Research
Ophiostoma europhioides and O. huntii are two very similar fungi known to cause blue-stain of timber. Both species have been isolated from pine and spruce in Canada and are characterized by Leptographium anamorphs and hat-shaped ascospores. The aim of this study was to compare isolates of O. huntii and O. europhioides from various parts of the-world. These isolates were compared morphologically using light and scanning electron microscopy. Sexual compatibility was determined using mating studies. O. huntii isolates from the different geographical areas were similar to each other but distinct from O. europhioides. A new Ophiostoma species, O. aenigmaticum, is described for the Japanese isolates; Leptographium aenigmaticum, its anamorph, is described as a new species.

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References (28)

Holoblastic phialides

Transactions of the British Mycological Society

Thallic phialides

Transactions of the British Mycological Society

The polyphyletic origins of ophiostomatoid fungi

Mycological Research

Differences in synchronisation of stages of conidial development in Leptographium species

Transactions of the British Mycological Society

Reclassification of Verticicladiella based on conidial development

Transactions of the British Mycological Society

Analysis of conidium ontogeny in anamorphs of Ophiostoma: Pesotum and Phialographium are synonyms of Graphium

Mycological Research

Ophiostoma novo-ulmi sp. nov., causative agent of current Dutch elm disease pandemics

Mycopathologia

A restudy of Ceratocystis penicillata and report of two American species of this genus from Europe

Mycologia

Ceratocystis versus Ophiostoma: a reappraisal

Mycologia

The evolution of insect-fungus relationships in the primary invasion of forest timber

Intercontinental epidemiology of Dutch elm disease

Annual Review of Phytopathology

Cycloheximide sensitivity as a taxonomic character in Ceratocystis

Mycologia

Leptographium root diseases on conifers

On the subdivision of Ceratocystis s.l., based on partial ribosomal DNA sequences

Canadian Journal of Botany

Cited by (21)

Taxonomy and pathogenicity of ophiostomatoid fungi associated with bark beetles infesting conifers in Japan, with special reference to those related to subalpine conifers

Phylogenetic relationship of Japanese isolates belonging to the Grosmannia piceiperda complex (Ophiostomatales)

Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs

New Leptographium species from Indonesia and Eastern North America

A new Leptographium species from Russia

Comparison of Ophiostoma huntii and O. europhioides and description of O. aenigmaticum sp. nov.