Elsevier

Matrix Biology

Volume 17, Issues 8–9, December 1998, Pages 615-623
Matrix Biology

Regular paper
Regulation of Osteopontin Gene Expression During Egg Shell Formation in the Laying Hen by Mechanical Strain

https://doi.org/10.1016/S0945-053X(98)90112-3Get rights and content

Abstract

The aim of this study is to evaluate the regulation of the osteopontin (OPN) gene expression by non-hormonal stimuli, such as calcium flux and mechanical strain during the daily egg cycle in the oviduct of the laying hen. After the egg enters the eggshell gland (ESG), the OPN gene is expressed by the epithelium cells in two waves: first by the basal cells and only then by the apical cells of the epithelium. A reduction in OPN gene expression was observed 1 h prior to laying. The calbindin gene, which marks the onset of calcification, was found to be expressed in the glandular epithelium starting 2 h after OPN gene expression. In addition, the formation of soft shells was accompanied by a reduction in calbindin, but not in OPN, gene expression. The application of a mechanical strain comparable to that induced by an egg led to induction of OPN gene expression at a normally quiescent phase in the cyclical expression of this gene. The induction of the gene was time- and strain-dependent and temporally similar to that induced by the entry of the egg into the ESG. In contrast, the calbindin gene was not affected by mechanical strain.

The ESG of the laying hen provides a system to study the effect of a mechanical strain on matrix protein production in vivo, in a relevant physiological setting. The finding suggests that, in contrast to calbindin, OPN gene expression is not regulated by calcium flux but rather by the mechanical strain imposed by the resident egg.

References (42)

  • C.W. Prince et al.

    1,25-dihydroxyvitamin D3 regulates the biosynthesis of osteopontin, a bone-derived cell attachment protein, in clonal osteoblast-like osteosarcoma cells

    Collagen Rel. Res.

    (1987)
  • K. Singh et al.

    Physiological properties and differential glycosylation of phosphorylated and non-phosphorylated forms of osteopontin secreted by normal rat kidney cells

    J. Biol. Chem.

    (1990)
  • B.H. Stemberger et al.

    Microscopic study of the initial stage of eggshell calcification

    Poult. Sci.

    (1977)
  • S. Striem et al.

    Modulation of quail intestinal and egg shell gland calbindin (Mr 28,000) gene expression by vitamin D3, 1,25-dihydroxyvitamin D3 and egg laying

    Mol. Cell. Endocrinol.

    (1991)
  • S. Weiner et al.

    Acidic macromolecules of mineralized tissues: The controllers of crystal formation

    Trends Biochem. Sci.

    (1991)
  • M. Wong et al.

    Collagen in the egg shell membrane of the hen

    Dev. Biol.

    (1984)
  • L. Addadi et al.

    A chemical model for the cooperation of sulfates and carboxylates in calcite crystal nucleation: relevance to biomineralization

  • A. Bar et al.

    Regulation of calbindin messenger RNA and calbindin turnover in intestine and shell gland of the chicken

    Am. J. Physiol.

    (1992)
  • W.T. Butler

    The nature and significance of osteopontin

    Connect. Tissue Res.

    (1989)
  • R.J. Etches

    The ovulatory cycle of the hen

    CRC Crit. Rev. Poult. Biol.

    (1990)
  • A. Faerman et al.

    Dramatic heterogeneity of transgene expression in the mammary gland of lactating mice: a model system to study the synthetic activity of mammary epithelial cells

    J. Histochem. Cytochem.

    (1995)
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