Optimization of conditions for conversion of tylosin to a novel antibiotic, acetyl-isovaleryl tylosin (AIV), by Streptomyces thermotolerans and scale-up to 200-liter pilot-scale fermentor

doi:10.1016/S0922-338X(97)82790-0

Journal of Fermentation and Bioengineering

Volume 84, Issue 1, 1997, Pages 77-81

Journal of Fermentat...

https://doi.org/10.1016/S0922-338X(97)82790-0 Get rights and content

Abstract

The rate of substrate (tylosin) supply, pH, dissolved oxygen concentration, and temperature for bioconversion of tylosin to acetyl-isovaleryl tylosin (AIV), were optimized in a flask and a 3-l jar fermentor. An AIV concentration 9.3 g/l was achieved when the pH was controlled at 7.0 and the tylosin supply rate was maintained at 200 mg/l/h during the period of bioconversion of tylosin to AIV after the cell growth phase. Based on the data obtained for the flask and jar-fermentor cultures, the bioconversion process was successfully scaled up to a 200-l fermentor. In this pilot-scale fermentor, 13.8 g/l of AIV was obtained at a yield of 83% based on the amount of tylosin supplied.

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Optimization of tylosin feeding rate profile in production of acetyl-isovaleryl tylosin (AIV) from tylosin by Streptomyces thermotolerans YN554
2001, Journal of Bioscience and Bioengineering
An optimal feed rate profile of a substrate (tylosin) for a novel antibiotic, acetyl-isovaleryl tylosin (AIV) production process was investigated. In the first step of optimization, a kinetic model for production of AIV from tylosin by Streptomyces thermotolerans was established properly using the least square method, followed by the confirmation that the proposed model could be used to predict the production process of AIV from tylosin. An objective function, state equations and an inequality constraint with respect to the tylosin feeding rate profile were applied to maximize the amount of AIV produced from tylosin in a fed-batch culture. The optimized tylosin feeding rate profile was determined using a direct iterative search algorithm based on the modified complex method. The simulation of AIV production at the optimal tylosin feeding profile indicates that the final amount of AIV is expected to be about 30% higher than that at the conventional constant tylosin feeding rate, which was also confirmed experimentally using a 30-l jar fermentor.
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Regular paperOptimization of conditions for conversion of tylosin to a novel antibiotic, acetyl-isovaleryl tylosin (AIV), by Streptomyces thermotolerans and scale-up to 200-liter pilot-scale fermentor

Abstract

The activity of 4″-acylated tylosin derivatives against macrolide-resistant gram-positive bacteria

J. Antibiot.

Screening for 16-membered macrolide-transforming microorganisms

J. Ferment. Bioeng.

Novel acyl derivatives of tylosin produced by microbial transformation

Regular paper
Optimization of conditions for conversion of tylosin to a novel antibiotic, acetyl-isovaleryl tylosin (AIV), by Streptomyces thermotolerans and scale-up to 200-liter pilot-scale fermentor