Polyamine Profiles of Gram-positive Catalase Positive Cocci

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Summary

Polyamine profiles of 26 type strains of Gram-positive, catalase-positive cocci, belonging to different phylogenetic lineages were obtained by gradient high performance liquid chromatography (HPLC) and fluorescence detection. Clearly, growth conditions affected the total amount of polyamines, but did not change the qualitative pattern of a culture. Hydrolysis with 6 N HCl revealed putrescine and cadaverine as bound polyamines of Staphylococcus kloosii DSM 20676T and S. auricularis DSM 20609T in addition to the pattern obtained by the usual extraction procedure with 0.2 N perchloric acid. Spermidine (SPD) and spermine (SPM), in different ratios, were the main polyamines in different species of Micrococcus, Dermacoccus, Kocuria, Kytococcus, Staphylococcus, Planococcus, Marinococcus, Kineococcus, Stomatococcus, and also Deinococcus. D. radiodurans displayed a unique profile with several unknown compounds. Nesterenkonia halobia was easily separated from the other cocci by the presence of cadaverine (CAD) as the main polyamine, Dermacoccus nishinomiyaensis by the presence of diaminopropane (DAP) as a major compound, and Kineococcus by the presence of putrescine (PUT) and cadaverine in addition to the major compounds SPD and SPM. Polyamines can be used for intrageneric differentiation within Micrococcus and Planococcus and allow also to distinguish S. auricularis and S. carnosus from other staphylococci.

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