Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes

doi:10.1016/S0278-6915(97)85468-3

Food and Chemical Toxicology

Volume 35, Issue 12, December 1997, Pages 1159-1164

https://doi.org/10.1016/S0278-6915(97)85468-3 Get rights and content

Abstract

Dried flower extracts of Hibiscus sabdarrifa L., a local soft drink material and medical herb, was found to possess antioxidant activity in the present study. In the preliminary studies, antioxidant potential of three fractions of the ethanol crude extract (HS-C: chloroform-soluble fraction; HS-E: ethyl acetate soluble fraction; HS-R: residual fraction) obtained from the dried flowers of Hibiscus sabdarrifa L. were evaluated by their capacity of quenching 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and inhibiting xanthine oxidase (XO) activity. HS-E showed the greatest capacity of scavenging free radical (EC₅₀ = 0.017 mg/ml), and HS-C showed the strongest inhibitory effect on XO activity (EC₅₀ = 0.742 mg/ml). Furthermore, antioxidant bioactivities of these crude extracts were investigated using a model of tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat primary hepatocytes. All fractions were found to inhibit significantly the unscheduled DNA synthesis (UDS) induced by t-BHP at a concentration of 0.20 mg/ml. HS-C and HS-E also decreased the leakage of lactate dehydrogenase (LDH) and the formation of malondialdehyde (MDA) induced by t-BHP (1.5 mm) considerably at a concentration of 0.10 and 0.20 mg/ml in the rat primary hepatocyte cultures. These results indicated that the dried flower extracts (HS-C and HS-E) of H. sabdarrifa L. protect rat hepatocytes from t-BHP-induced cytotoxicity and genotoxicity by different mechanisms.

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Citation Excerpt :
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Cette étude a évalué le potentiel antidiabétique de la fraction aqueuse riche en flavonoïdes d’un extrait méthanolique d’Hibiscus sabdariffa calyx (HSCE) sur les cellules β du pancréas de rats modèles diabétiques de type I expérimental. Le diabète sucré de type 1 a été induit chez le rat Wistar par une injection intrapéritonéale unique de 80 mg/kg de poids corporel de streptozotocine (STZ) dissoute dans un tampon citrate 0,1 M (pH 6,3). Les rats ont été divisés en cinq groupes (n = 12) comprenant le groupe témoin normal, le groupe test I, le contrôle négatif diabétique, le groupe test II et le contrôle positif diabétique. Les groupes tests ont reçu 1,75 g/kg de HSCE par gavage pendant 15 jours. Les animaux ont été sacrifiés; la région splénique du pancréas et leur sérum ont été évaluée pour les paramètres histopathologiques et biochimiques, respectivement. Les résultats de l’analyse histopathologique et les paramètres biochimiques sériques ont mis en évidence des effets régénérateurs de l’extrait sur les dommages causés aux cellules β par le STZ. Les taux réduits de glutathion, de catalase et de superoxyde dismutase dans le sérum de rats diabétiques ont été significativement améliorés chez les rats traités à H. sabdariffa (p < 0,05). L’examen histologique de coupes histologiques a montré des modifications dégénératives et nécrotiques des îlots pancréatiques de Langerhans, une dégranulation des cellules β, des noyaux pycnotiques dans les cellules β, une diminution de la densité cellulaire des îlots et une vacuolisation sévère dans les îlots du groupe de rats diabétique contrôle traités par STZ. La morphologie du pancréas de rats diabétiques traités avec du HSCE (groupe test II) a révélé des améliorations remarquables dans les îlot de Langerhans. Des études stéréologiques ont également révélé que le traitement par HSCE améliorait le volume des îlots pancréatiques et la densité numérique de cellules β (nombre de cellules β par unité d’îlot) appauvries par le diabète STZ. Nous concluons que le mécanisme antidiabétique potentiel de Hibiscus sabdariffa dans le diabète STZ passe par l’induction de la régénération des cellules β et de son fort potentiel antioxydant.
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Research sectionProtective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes

Abstract

Mutation Research

Toxicology and Applied Pharmacology

Biochimica et Biophysica Acta

Cancer Letters

Free Radical Biology and Medicine

Chemistry and Physics of Lipids

Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases

Science

Primary monolayer cultures of adult rat liver parenchymal cells suitable for study of regulation of enzyme synthesis

In Vitro

Molecular mechanism of oxygen radical carcinogenesis and mutagenesis: the role of DNA base damage

Molecular Carcinogenesis

Proxident states and tumor promotion

Science

Inhibitory effects of flavonoids on xanthine oxidase

Anticancer Research

Research section
Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes