Immunocytochemical Demonstration of Simultaneous Synthesis of Types I, III and V Collagen and Fibronectin in Mouse Embryonic Palatal Mesenchymal Cells In Vitro

https://doi.org/10.1016/S0174-173X(87)80026-2Get rights and content

Abstract

An immunocytochemical study was made on the palatal mesenchymal cells obtained from mouse embryos during palatal development with special reference to synthesis of types of collagen and fibronectin in vitro. Most cells showed positive staining with antibodies against the four proteins examined. The staining for type I collagen was most intense among the four proteins and was distributed perinuclearly. The staining for type III collagen was quite similar as that for type I collagen but less intense, whereas that for type V collagen was weak and its staining pattern was different from those for types I and III collagen in that the surface of the plasma membrane, in addition to the perinuclear cytoplasm, showed weak staining for type V collagen. Antibodies to fibronectin showed perinuclear and extracellular fibrous staining. These data suggest that palatal mesenchymal cells synthesize types I, III, and V collagen and fibronectin simultaneously.

References (32)

  • M.H. Silver et al.

    Epidermal growth factor stimulates type-V collagen synthesis in cultured murine palatal shelves

    Differentiation

    (1984)
  • V.-J. Uitto et al.

    Effect of hydrocortisone on collagen synthesis in cultured mouse palatal explants

    Arch. Oral Biol.

    (1979)
  • E.K. Bayne et al.

    Extracellular matrix organization in developing muscleccc Correlation with acetylcholine receptor aggregates

    J. Cell Biol.

    (1984)
  • R.E. Burgeson et al.

    Fetal membrane collagensccc Identification of two new collagen alpha chains

    Proc. Natl. Acad. Sci. USA

    (1976)
  • J.M. Burke et al.

    Synthesis of type I and III procollagen and collagen by monkey aortic smooth muscle cells in vitro

    Biochemistry

    (1977)
  • J.M. Foidart et al.

    Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line

    Lab. Invest

    (1980)
  • Cited by (0)

    a

    Dr. K. Kurisu, Second Department of Anatomy, Faculty of Dentistry 61, Kyushu University, Maidashi, Higashi-Ku, Fukuoka 812, Japan.

    View full text