Trends in Genetics
Research updateEvidence for cysteine clustering in thermophilic proteomes
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Higher cysteine clustering in thermophilic proteomes
What is the significance of the extremely high cysteine clustering observed in Methanobacterium and Pyrococcus? After an inspection of several physiological parameters, we turned our attention to their growth temperatures: Saccharomyces cerevisiae and E. coli grow optimally at 30°C and 37°C, respectively, whereas Methanobacterium and Pyrococcus grow optimally at 65°C and 103°C, respectively.
To test the hypothesis that cysteine clustering is influenced by growth temperature, we defined a
Amino acid clustering and proteome evolution
On a percent basis, the Pyrococcus proteome contains about half the number of cysteines (0.55%) found in the E. coli proteome (1.17%) (see data on website). The higher amount of cysteine clustering found in Pyrococcus is due to the fact that, with increasing growth temperature, the number of isolated cysteines decreases rapidly, whereas the number of clustered cysteines, if anything, increases slightly (Table 3). This observation can be rationalized assuming that, for some reason, isolated
Acknowledgements
We thank Giovanni Lavorgna for useful suggestions and comments on the manuscript.
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