Elsevier

Virus Research

Volume 56, Issue 2, August 1998, Pages 125-133
Virus Research

Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen

https://doi.org/10.1016/S0168-1702(98)00035-5Get rights and content

Abstract

In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3Dpol by PBMC and CD4+ T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VP1 was recognised in an homotypic context whereas VP2, VP3, VP4 and P3Dpol were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3Dpol was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3Dpol in purified virus. Thus, P3Dpol is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine.

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Acknowledgements

We thank Dr A. Sanderson for the gift of neonatal rabbit serum. M.G.-V. was the recipient of a postgraduate studentship from the Institute for Animal Health and M.F. was the recipient of an AFRC studentship.

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