Cryopreservation of turbot () spermatozoa
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Cited by (85)
Male reproductive potential in the Black Sea turbot (Scophthalmus maximus) spawning populations
2022, Fisheries ResearchA step towards using the semen of the common pandora Pagellus erythrinus for practical applications of cryo-research: The computer-assessed sperm motility pattern in long-term cryostored samples
2020, AquacultureCitation Excerpt :Common pandora semen samples cryopreserved according to our protocols proved to retain the ability of activation for a considerable time after thawing; in fact no or scarce differences were obtained between semen samples activated immediately and 3 h after thawing; moreover in some cases significantly higher TM percentages and VCL and DNC values were recorded when samples were activated after a 3 h-incubation in the extender, at 18 °C. An increase in sperm motility parameters after incubation in an immobilizing medium mimicking seminal plasma has been observed for common carp (Linhart et al., 2008; Cejko et al., 2019) and brown trout (Horváth et al., 2015); the resting period at room temperature, together with the ionic composition of the diluents, have been supposed to allow the ATP reload by mitochondrial metabolism in both fresh (Linhart et al., 2008; Alavi et al., 2019) and frozen/thawed (Dreanno et al., 1997) sperm cells. We can therefore infer that all the here tested protocols were able to preserve the integrity of the metabolic pathways involved in the P. erythrinus sperm motility, also after a long-term storage.
Proteomic identification of seminal plasma proteins related to the freezability of carp semen
2017, Journal of ProteomicsOptimization of conditions for the cryopreservation of yellow catfish (Pelteobagrus fulvidraco) sperm
2017, CryobiologyCitation Excerpt :As in the case of European catfish, DMA was determined as better cryoprotectant than Me2SO or MeOH in sucrose solution [33], but Me2SO yielded higher post-thaw sperm motility in a saline-based solution [27]. As a critical factor in sperm cryopreservation, optimal freezing rate showed a great degree of variation among different species, from 5 °C/min for silver barb [48] and 40 °C/min for Persian sturgeon [2] to 99 °C/min for turbot [10]. Apart from differences in lipid composition of plasma membranes [23], ability of sperm cells to regulate osmotic pressure also has a effect on optimal freezing rate during cryopreservation [12].