Analysis of mitochondrial translation products in Vivo and in organello in yeast
References (19)
- et al.
The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria
Cell
(1996) - et al.
Oxa1p mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space
FEBS Lett.
(1997) - et al.
Proteolytic breakdown of membrane-associated polypeptides in mitochondria of Saccharomyces cerevisiae
Methods Enzymol.
(1995) - et al.
Mitochondrial gene expression in Saccharomyces cerevisiae. I. optimal conditions for protein synthesis in isolated mitochondria
J. Biol. Chem.
(1984) - et al.
Yta10p is required for the ATP-dependent degradation of polypeptides in the inner membrane of mitochondria
FEBS Lett.
(1994) - et al.
The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease
EMBO J.
(1998) - et al.
Biogenesis of mitochondria
Annu. Rev. Cell. Biol.
(1988) - et al.
The unusual Var1 gene of yeast mitochondrial DNA
Science
(1985) - et al.
Control of mitochondrial gene expression in Saccharomyces cerevisiae
Annu. Rev. Genet.
(1990)
Cited by (33)
Rcf proteins and their differential specificity for respiratory chain complexes: A unique role for Rcf2 on oxygen sensitive supercomplexes?
2021, Biochimica et Biophysica Acta - Molecular Cell ResearchThe Cox1 C-terminal domain is a central regulator of cytochrome c oxidase biogenesis in yeast mitochondria
2017, Journal of Biological ChemistryCitation Excerpt :Western blots were probed with antibodies against HA (Roche Applied Science), Myc (Roche Applied Science), Cox1, Coa3 (A. Barrientos), Cox2 (Mitosciences), Cox3 (Mitosciences), and citrate synthase. In vivo pulse labeling of cells or in organello labeling of crude mitochondria with [35S]Methionine was performed as described previously (51). The radiolabeled proteins were separated by SDS-PAGE, and the gel was dried before analysis with a Typhoon 8600 PhosphorImager (GE Healthcare).
Bcs1p can rescue a large and productive cytochrome bc<inf>1</inf> complex assembly intermediate in the inner membrane of yeast mitochondria
2011, Biochimica et Biophysica Acta - Molecular Cell ResearchCitation Excerpt :The reaction was initiated by adding the ubiquinol analog, decylubiquinol (DBH2), to a final concentration of 50 μM, and the rate of cytochrome c reduction was calculated from the absorbance increase at 550 nm. Mitochondrial translation products were specifically labelled using previously employed methods, with minor modifications [26–28]. Yeast cells were grown in the minimal medium YNB-U to an OD578 of 0.5 to 2.0.
The carboxyl-terminal end of Cox1 is required for feedback assembly regulation of Cox1 synthesis in Saccharomyces cerevisiae mitochondria
2010, Journal of Biological ChemistryCitation Excerpt :The radiolabeled proteins were separated on a 16% polyacrylamide gel and transferred to a PVDF membrane before they were analyzed with a Typhoon 8600 Phosphorimager (GE Healthcare). Translation in isolated mitochondria (3 mg of protein/ml) in the presence of [35S]methionine was performed as previously described (32). After translation, mitochondria were washed with 0.6 m sorbitol, 20 mm HEPES, pH 7.4, and lysed with a buffer containing 100 mm NaCl, 20 mm Tris, pH 7.4, and either 1% digitonin (w/v) or 1% dodecyl maltoside (w/v).
Yeast AEP3p is an accessory factor in initiation of mitochondrial translation
2009, Journal of Biological ChemistryCitation Excerpt :Cells were grown to mid-log phase, harvested, and washed with minimal galactose medium (0.7% yeast nitrogen base, 2% galactose) without amino acids. In vivo labeling of mitochondrial translation products using [35S]methionine in the presence of cytoplasmic protein synthesis inhibitor cycloheximide was performed as described previously (36). Translation products were separated by SDS-PAGE using gels (15% acrylamide, 0.1% bisacrylamide) prepared as described (36).