Flow cytometric analysis of DNA content in budding yeast
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Cited by (81)
Sulfide-quinone oxidoreductase is required for cysteine synthesis and indispensable to mitochondrial health
2021, Redox BiologyCitation Excerpt :The survival rate was determined by calculating the ratio of Δhmt2 CFU to wt CFU. The classic PI staining method was used to analyze cell cycles of S. pombe cells as previously reported [32]. Briefly, log-phase cells were grown to OD600 of 0.8–1.0, then harvested and washed two times, and diluted to OD600 of 0.1 in EMM medium.
Essential Oils of Rosmarinus officinalis L., Cymbopogon citratus (DC.) Stapf., and the phyto-compounds, delta-carene and alpha-pinene mediate cell cycle arrest at G2/M transition in budding yeast Saccharomyces cerevisiae
2021, South African Journal of BotanyCitation Excerpt :The percentage of cells under the various phases of budding (as described above) was plotted against the respective time intervals (2 h, 4 h, 6 h, 8 h and 24 h). The yeast sample preparation was carried out as described by Haase and Lew (1997). Briefly, yeast cells (0.25 ml of ~ 0.5 A600) incubated in the absence (control) or presence of R. officinalis essential oil for 24 h were harvested by centrifugation, resuspended by vortexing and fixed with 1 ml of cold ethanol (−20 °C) for 3 h.
Lack of superoxide dismutase in a rad51 mutant exacerbates genomic instability and oxidative stress-mediated cytotoxicity in Saccharomyces cerevisiae
2018, Free Radical Biology and MedicineCell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p
2011, Journal of Biological ChemistryCitation Excerpt :The samples were stained overnight in 10 μg/ml propidium iodide as described previously (16). DNA content of samples was analyzed as described by Haase and Lew (30) on a Cell Lab QuantaTM SC flow cytometer (Beckman Coulter Australia, Gladesville, Australia). To detect the presence of cysteines converted to sulfenic acid residues, cells expressing Swi6p from the glyceraldehyde-3-phosphate dehydrogenase (TDK3) promoter were grown in SC-Ura to A600 of 0.4 and split into equal aliquots for treatment with and without LoaOOH (30 μm) for 5 min.