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Thiamine Deficiency: An Important Consideration in Critically Ill Patients
2018, American Journal of the Medical SciencesCitation Excerpt :Thiamine and TMP are found in plasma; thiamine, TMP, TDP and TTP are found in erythrocytes and other tissues. Approximately 1% of total body thiamine is in whole blood, and 80% of whole blood thiamine is in erythrocytes.7 Gangolf et al8 measured thiamine and its phosphorylated derivatives in multiple biopsies from human tissues.
A single-step method for simultaneous quantification of thiamine and its phosphate esters in whole blood sample by ultra-performance liquid chromatography-mass spectrometry
2018, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :The method by determining erythrocyte transketolase activity only quantifies TDP content, and has poor inter-assay precision and sample storage stability because of rapid inactivation of the transketolase [26]. HPLC methods can be used for simultaneous determination of thiamine and its phosphate esters in human whole blood [17,25,27–32]. However, current HPLC methods required pre- or post-column fluorescence derivatization to improve sensitivity.
Up-regulation of vitamin B1 homeostasis genes in breast cancer
2013, Journal of Nutritional BiochemistryCitation Excerpt :Thus, the relative expression of PDH, TKT and α-KGDH may vary depending on the metabolic phenotype of the cell and be an important contributor to the intracellular level of TPP. Additionally, TMP can be produced from the dephosphorylation of TPP by thiamine pyrophosphatase; TMP is also found extracellularly at levels approximating those of free thiamine [41,45,46]. The origin of TMP in the plasma has been suggested to be from the efflux of TMP out of the cells by the reduced folate transporter encoded by the SLC19A1 gene [45,47].
Determination of thiamin diphosphate in whole blood samples by high-performance liquid chromatography-A method suitable for pediatric diagnostics
2009, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesBiosynthesis and uptake of thiamine (vitamin B<inf>1</inf>) in bloodstream form Trypanosoma brucei brucei and interference of the vitamin with melarsen oxide activity
2006, International Journal for ParasitologyThe analysis of thiamin and its derivatives in whole blood samples under high pH conditions of the mobile phase
2005, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences