Synthesis of fluorescent probes for the detection of abasic sites in DNA
The title molecules 1–3 have been prepared to titrate a major DNA lesion, the abasic site.
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Site-specific unnatural base excision via visible light
2022, Chemical CommunicationsSensitive detection of abasic sites in double-stranded DNA based on the selective reaction of enzymes
2022, Analytica Chimica ActaCitation Excerpt :However, the specificity of the method was not satisfactory because of the cross-reaction of antibodies. The covalent chemical probe method can be divided into O-(pyridin-3-methyl) hydroxylamine (PMOA) probes and aldehyde reaction probes (ARP) [17]. The former was often combined with the LC-MS/MS method [18], and its detection results were accurate and reliable.
Polysialylation of human coagulation factor VIII
2019, Polymer-Protein Conjugates: From Pegylation and BeyondDetection of abasic DNA by means of impedance spectroscopy
2018, Electrochemistry CommunicationsCitation Excerpt :Based on this principle several attempts have been made to improve the detection system. For example, by using the fluorescent probe Lissamine-Rhodamine B the sensitivity reached 1 a-site in 105 nucleotides [8]. Alternative systems use the FRET effect based on fluorescein-based probes [4], atomic force microscopy to detect avidin binding to the ARP [9], or modifications of ARP to get a better pH stability and sensitivity [10].
A chemical toxicity sensor based on the electrochemiluminescence quantification of apurinic/apyrimidinic sites in double-stranded DNA monolayer
2017, Sensors and Actuators, B: ChemicalCitation Excerpt :Kojima et al. improved the reaction rate of the probe by incorporating a hydrophobic and a hydrophilic residue into an aminooxy group [19]. In addition to the biotin tags described above, fluorescent dye-hydroxylamine conjugates were also synthesized as covalent probes, which were measured directly for AP site detection [20–22]. In a different approach, osmium tetroxide-2,2′-bipyridine was employed as an electrochemical probe of AP sites, which reacts covalently with the unpaired thymine bases in damaged DNA [23].
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