Elsevier

Metabolism

Volume 13, Issue 10, Part 2, October 1964, Pages 1043-1058
Metabolism

Synthesis of biologically active peptides related to ACTH

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Summary

The various protecting agents for the ∈-NH2 group of lysine and the guanidine group of arginine have been discussed with emphasis on the use of tosylation. The effectiveness of sodium in liquid ammonia for the removal of protecting groups has also been discussed. The synthesis of α1–10 + 15–19-ACTH, α1–17-ACTH and α1–19-ACTH has been presented. The homogeneity of synthetic products was established by chromatography on carboxylmethylcellulose, disc electrophoresis on Polyacrylamide gel and amino acid analysis. The biological activities of these synthetic peptides, with respect to adrenal-stimulation, melanocyte-stimulation, and lipolysis were summarized. It was found that a separation of these three biological activities has been achieved.

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    1

    From the Hormone Research Laboratory, University of California, Berkeley, Calif.

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