Gas chromatographic determination of β-phenethylbiguanide and its metabolite p-hydroxy-β-phenethylbiguanide in serum and urine

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Abstract

A gas chromatographic method is presented for the detection of β-phenethylbiguanide (PEBG) and its metabolite, p-hydroxy-β-phenethylbiguanide (p-OH PEBG). The procedure is applicable for the determination of the drug and its metabolite in the serum and urine of rats. The detection limit is 0.2 μg PEBG and 0.5 μg p-OH PEBG per ml of serum or urine.

A time course study of blood concentration and elimination rate following intraperitoneal injection of 100 mg/kg of PEBG to normal rats was performed. β-PEBG was found to be present in the blood and the urine, p-OH PEBG was only detected in the urine. Twenty-four hours following intraperitoneal injection, the urine contained 32% of the administered dose, 20% as unaltered PEBG and 12% as p-OH PEBG.

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Portions of this work were submitted by M.M. in partial fulfilment of the requirements of the Master of Science degree, August 1974

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