Incorporation of sulphate in peritoneal mast cells isolated from young and adult rats - ScienceDirect

Experimental Cell Research

Volume 33, Issues 1–2, January 1964, Pages 254-263

Experimental Cell Research

https://doi.org/10.1016/S0014-4827(64)81031-4 Get rights and content

Summary

1.
The incorporation of ³⁵S-labeled sulphate into peritoneal mast cells from young and adult rats has been studied in vitro with the aid of autoradiographic and microphotographic techniques.
2.
The appearance of polysaccharide-bound ³⁵S-sulphate into mast cells is a positive function of the incubation time within a 4-hr period.
3.
The rate of incorporation is faster in mast cells from young than adult animals either when referred to cell unit-mass or when expressed on a per cell basis.
4.
When referred to cell unit-mass, sulphate incorporation behaves as a negative linear function of mast cell size, the negative gradient being greater in mast cells from young than from adult rats.
5.
The data suggest that real differences exist between mast cells of young and adult animals and that these differences depend upon factors inherent in the individual cells (cell senescence) and factors linked to the animal as a whole (ageing of the host).

The significance of these observations with respect to the mode of action of these factors is briefly discussed.

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¹: This work was supported by a grant from Comitato Nazionale Energia Nucleare, Roma, Italy.

Copyright © 1964 Published by Elsevier Inc.